Perforin Antibody (deltaG9)

Images

 
Immunocytochemistry/ Immunofluorescence: Perforin Antibody (deltaG9) [NBP1-45774] - Isolated CD8 T-cells showing intracellular perforin (green).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
deltaG9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Perforin Antibody (deltaG9) Summary

Immunogen
Purified granules from human YT lymphoma cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rabbit
Gene
PRF1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Immunoprecipitation (2-8 ug/ml), Flow cytometry (2-8 ug/ml), Immunohistochemistry (2-8 ug/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 ug/ml). Optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-45774 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1.0 mg/ml BSA, 50% Glycerol
Preservative
Thimerosal
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Perforin Antibody (deltaG9)

  • Cytolysin
  • FLH2
  • HPLH2
  • HPLH2lymphocyte pore forming protein
  • Lymphocyte pore-forming protein
  • MGC65093
  • P1PFN1
  • perforin 1 (pore forming protein)
  • perforin-1
  • PFPcytolysin

Background

This antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perforin Antibody (NBP1-45774) (0)

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Review for Perforin Antibody (NBP1-45774) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-45774:
Filter by Applications
ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Perforin NBP1-45774
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reviewed by:
Preet Kumar
ICC Human 05/10/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedIsolated CD8 cells
SpeciesHuman
LotA40888

Comments

CommentsThe green inside the cells represents perforin

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Perforin Antibody (NBP1-45774) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Perforin Antibody (NBP1-45774)

Discover related pathways, diseases and genes to Perforin Antibody (NBP1-45774). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Perforin Antibody (NBP1-45774)

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Research Areas for Perforin Antibody (NBP1-45774)

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Blogs on Perforin.

Perforin a Protective Serial Killer
Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Perforin Antibodies for Detecting Immune System Diseases
 Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Recent Reviews

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1
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Preet Kumar
05/10/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol PRF1
Entrez