PEPD Antibody (1D5-H3) - Azide and BSA Free

Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.Lane 1: PEPD transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of monoclonal antibody to PEPD on HepG2 cell. Antibody concentration 10 ug/ml.

Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - PEPD monoclonal antibody (M01), clone 1D5-H3 Analysis of PEPD expression in HepG2.

Immunoprecipitation: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of PEPD transfected lysate using anti-PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD MaxPab rabbit polyclonal antibody.

Sandwich ELISA: PEPD Antibody (1D5-H3) [H00005184-M01] - Detection limit for recombinant GST tagged PEPD is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 1D5-H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PEPD Antibody (1D5-H3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PEPD (AAH15027, 1 a.a. ~ 493 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVAEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQEPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK
• Specificity: PEPD - peptidase D
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PEPD
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PEPD Antibody (1D5-H3) - Azide and BSA Free

• aminoacyl-L-proline hydrolase
• EC 3.4.13.9
• Imidodipeptidase
• MGC10905
• peptidase Dimidodipeptidase
• PRD
• PROLIDASE
• Proline dipeptidase
• xaa-Pro dipeptidase
• X-Pro dipeptidase

Background

Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of iminoacids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEPD Antibody (H00005184-M01)(1)

Publication using H00005184-M01

• Olivares O, Mayers JR, Gouirand V et al. Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions Nat Commun 2017-07-07 [PMID: 28685754] (WB, Human)

Bioinformatics

• Gene Symbol: PEPD
• Entrez:
  • 5184(Human)
• OMIM:
  • 170100(Human)
• Uniprot:
  • AAH15027(Human)