PEPD Antibody (1D5-H3)

Images

 
Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - PEPD monoclonal antibody (M01), clone 1D5-H3 Analysis of PEPD expression in HepG2.
Immunocytochemistry/ Immunofluorescence: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of monoclonal antibody to PEPD on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.Lane 1: PEPD transfected lysate (Predicted MW: 54.6 ...read more
Immunoprecipitation: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of PEPD transfected lysate using anti-PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD MaxPab rabbit polyclonal ...read more
Sandwich ELISA: PEPD Antibody (1D5-H3) [H00005184-M01] - Detection limit for recombinant GST tagged PEPD is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
1D5-H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PEPD Antibody (1D5-H3) Summary

Immunogen
PEPD (AAH15027, 1 a.a. - 493 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVAEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQEPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK
Specificity
PEPD - peptidase D
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PEPD
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Publications
Read Publication using
H00005184-M01 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PEPD Antibody (1D5-H3)

  • aminoacyl-L-proline hydrolase
  • EC 3.4.13.9
  • Imidodipeptidase
  • MGC10905
  • peptidase Dimidodipeptidase
  • PRD
  • PROLIDASE
  • Proline dipeptidase
  • xaa-Pro dipeptidase
  • X-Pro dipeptidase

Background

Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of iminoacids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PEPD Antibody (H00005184-M01)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PEPD
Entrez
OMIM
Uniprot