PEG10 Overexpression Lysate

Western Blot: PEG10 Overexpression Lysate (Adult Normal) [NBL1-14280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PEG10.

• Reactivity: Hu
• Applications: WB

PEG10 Overexpression Lysate Summary

• Description:

  PEG10 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC208683
  
  Accession#: NM_001040152
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: PEG10

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 36.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for PEG10 Overexpression Lysate

• EDR
• embryonal carcinoma differentiation regulated
• Embryonal carcinoma differentiation-regulated protein
• HB-1
• KIAA1051EDR
• Mammalian retrotransposon-derived protein 2
• Mar2
• Mart2
• MEF3 Like 1
• MEF3L
• MEF3L1
• Myelin expression factor 3-like protein 1
• paternally expressed 10
• Paternally expressed gene 10 protein
• PEG10
• retrotransposon gag domain containing 3
• Retrotransposon gag domain-containing protein 3
• Retrotransposon-derived gag-like polyprotein
• retrotransposon-derived protein PEG10
• RGAG3
• RGAG3MEF3-like protein 1
• RTL2
• SIRH1
• Ty3/Gypsy-like protein

Background

This gene includes two overlapping reading frames of the same transcript encoding distinct isoforms. The shorter isoform has a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons, and it functions in part by interacting with members of the TGF-beta receptor family. The longer isoform has the active-site DSG consensus sequence of the protease domain of pol proteins. The longer isoform is the result of -1 translational frameshifting that is also seen in some retroviruses. Expression of these two isoforms only comes from the paternal allele due to imprinting. Increased gene expression (as observed by an increase in mRNA levels) is associated with hepatocellular carcinomas. [provided by RefSeq]. Transcript Variant: This variant (1) includes two overlapping reading frames - RF1 and RF1/2. The shorter isoform (RF1) is encoded from RF1, which has an identical N-terminal sequence, as compared to isoform RF1/RF2. This isoform RF1 is made when -1 translational frameshifting does not occur. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PEG10