PDLIM5 Antibody (3E11-F6) - Azide and BSA Free

Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - PDLIM5 monoclonal antibody (M01), clone 3E11-F6. Analysis of PDLIM5 expression in HepG2.

Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Analysis of monoclonal antibody to PDLIM5 on A-431 cell. Antibody concentration 10 ug/ml.

Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Western Blot analysis of PDLIM5 expression in A-431 ( Cat # L015V1 ).

Sandwich ELISA: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Detection limit for recombinant GST tagged PDLIM5 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3E11-F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PDLIM5 Antibody (3E11-F6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PDLIM5 Antibody (3E11-F6) - Azide and BSA Free (H00010611-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-PDLIM5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PDLIM5 (AAH08741, 1 a.a. ~ 596 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKAAQANVRIGDVVLSIDGINAQGMTHLEAQNKIKGCTGSLNMTLQRASAAPKPEPVPVQKGEPKEVVKPVPITSPAVSKVTSTNNMAYNKAPRPFGSVSSPKVTSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKKRLIEDTEDWRPRTGTTQSRSFRILAQITGTEHLKESEADNTKKANNSQEPSPQLASSVASTRSMPESLDSPTSGRPGVTSLTTAAAFKPVGSTGVIKSPSWQRPNQGVPSTGRISNSAAYSGSVAPANSALGQTQPSDQDTLVQRAEHIPAGKRTPMCAHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYEKFFAPECGRCQRKILGEVINALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICHGCEFPIEAGDMFLEALGYTWHDTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF
• Specificity: PDLIM5 - PDZ and LIM domain 5
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDLIM5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDLIM5 Antibody (3E11-F6) - Azide and BSA Free

• Enh
• ENH1
• ENHLIM
• Enigma homolog
• enigma-like LIM domain protein
• Enigma-like PDZ and LIM domains protein
• PDZ and LIM domain 5
• PDZ and LIM domain protein 5

Background

The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDLIM5 Antibody (H00010611-M01)(1)

Publication using H00010611-M01

• Xiaolan H, Rongmei Q, Yan P et al. Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments. Biomolecules. 2021-05-19 [PMID: 34069539]

Bioinformatics

• Gene Symbol: PDLIM5
• Entrez:
  • 10611(Human)
• OMIM:
  • 605904(Human)
• Uniprot:
  • AAH08741(Human)