PDK2 Antibody (JB66-93)

Western Blot: PDK2 Antibody (JB66-93) [NBP2-75611] - Analysis of PDK2 on rat heart tissue lysate using anti-PDK2 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: PDK2 Antibody (JB66-93) [NBP2-75611] - Analysis of paraffin-embedded rat brain tissue using anti-PDK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: PDK2 Antibody (JB66-93) [NBP2-75611] - Analysis of SH-SY-5Y cells with PDK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: PDK2 Antibody (JB66-93) [NBP2-75611] - Analysis of paraffin-embedded human colon cancer tissue using anti-PDK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PDK2 Antibody (JB66-93) [NBP2-75611] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-PDK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PDK2 Antibody (JB66-93) [NBP2-75611] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-PDK2 antibody. Counter stained with hematoxylin.

Product Discontinued

PDK2 Antibody (JB66-93) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PDK2 aa 358-407 / 407. (SwissProt: Q15119 Human)
• Localization: Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PDK2 Antibody (JB66-93)

• EC 2.7.11
• EC 2.7.11.2
• lipoamide, kinase isozyme 2, mitochondrial
• PDH kinase 2
• PDHK2
• PDK2
• PDKII
• Pyruvate dehydrogenase kinase isoform 2
• pyruvate dehydrogenase kinase, isoenzyme 2
• pyruvate dehydrogenase kinase, isozyme 2

Background

PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDK2 Antibody (NBP2-75611)(1)

Publication using NBP2-75611

• Tiersma JF, Evers B, Bakker BM et al. Pyruvate Dehydrogenase Kinase Inhibition by Dichloroacetate in Melanoma Cells Unveils Metabolic Vulnerabilities International journal of molecular sciences [PMID: 35409102]

Bioinformatics

• Gene Symbol: PDK2