Recombinant Mouse PDGFA Protein

ELISA: PDGFA Protein [NBP1-43148] - Proliferation of 3T3 cells in response to Mouse PDGF-AA Recombinant Protein

• Reactivity: Mu
• Applications: ELISA, Bioactivity
• Concentration: 0.1 mg/ml

Recombinant Mouse PDGFA Protein Summary

• Description: Recombinant non-glycosylated homodimer of 126 amino acids corresponding to PDGF.

  Source: E. coli

• Details of Functionality: This reagent has been tested in bioassays using the mouse cell line 3T3. The ED50 measured in a 3T3 proliferation assay is typically 10 ng/ml, corresponding to a specific activity of approximately 1 x10^5Units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDGFA
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Immunoassay
• Theoretical MW: 28.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Mouse PDGFA Protein

• PDGF-1
• PDGF1PDGF A-chain
• PDGF-A
• Platelet-derived growth factor A chain
• platelet-derived growth factor alpha chain
• platelet-derived growth factor alpha isoform 2 preproprotein
• platelet-derived growth factor alpha polypeptidePDGF subunit A
• platelet-derived growth factor subunit A

Background

The platelet-derived growth factor AA (PDGF-AA) is a member of the PDGF family. The PDGF proteins are derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB). PDGF-AA plays an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in e.coli is a non-glycosylated homodimer of 126 amino acids with a molecular weight of 28.9 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDGFA