Human PDGFA ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1758
    • Availability
      Product Discontinued

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Human PDGFA ELISA Kit (Colorimetric) Summary

Sensitivity
<40 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PDGFA

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
PDGFA (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGFA.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent (Item E2): 5x concentrated buffer.
  2. Detection Antibody PDGF-AA (Item F): Biotinylated anti-human PDGF-AA.Each vial is enough to assay half the microplate.
  3. HRP-Streptavidin concentrates (Item G): 200x concentratedHRP-conjugated Streptavidin.
  4. PDGF-AA Microplate (Item A): Coated with anti-human PDGF-AA
  5. Standard Protein (Item C): Human PDGF-AA. 1 vial is enough to run eachstandard in duplicate.
  6. Stop Solution (Item I): 0.2 M sulfuric acid.
  7. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
  8. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human PDGFA ELISA Kit (Colorimetric)

  • PDGF-1
  • PDGF1PDGF A-chain
  • PDGF-A
  • Platelet-derived growth factor A chain
  • platelet-derived growth factor alpha chain
  • platelet-derived growth factor alpha isoform 2 preproprotein
  • platelet-derived growth factor alpha polypeptidePDGF subunit A
  • platelet-derived growth factor subunit A

Background

Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. This antibody is specific for B form and reacts with PDGF(AB) and PDGF(BB) protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for PDGFA ELISA Kit (KA1758). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol PDGFA