Human PDGFA ELISA Kit (Colorimetric) Summary
| Sensitivity |
<40 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
PDGFA |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
PDGFA (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGFA. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent (Item E2): 5x concentrated buffer.
- Detection Antibody PDGF-AA (Item F): Biotinylated anti-human PDGF-AA.Each vial is enough to assay half the microplate.
- HRP-Streptavidin concentrates (Item G): 200x concentratedHRP-conjugated Streptavidin.
- PDGF-AA Microplate (Item A): Coated with anti-human PDGF-AA
- Standard Protein (Item C): Human PDGF-AA. 1 vial is enough to run eachstandard in duplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human PDGFA ELISA Kit (Colorimetric)
Background
Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. This antibody is specific for B form and reacts with PDGF(AB) and PDGF(BB) protein.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for PDGFA ELISA Kit (KA1758). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.