Recombinant Mouse PDGFA Protein

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ELISA: PDGFA Protein [NBP1-43148] - Proliferation of 3T3 cells in response to Mouse PDGF-AA Recombinant Protein

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IA
Concentration
0.1 mg/ml

Order Details

Recombinant Mouse PDGFA Protein Summary

Description
Recombinant non-glycosylated homodimer of 126 amino acids corresponding to PDGF.

Source: E. coli

Details of Functionality
This reagent has been tested in bioassays using the mouse cell line 3T3. The ED50 measured in a 3T3 proliferation assay is typically 10 ng/ml, corresponding to a specific activity of approximately 1 x10^5Units/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PDGFA
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Mouse PDGFA Protein

  • PDGF-1
  • PDGF1PDGF A-chain
  • PDGF-A
  • Platelet-derived growth factor A chain
  • platelet-derived growth factor alpha chain
  • platelet-derived growth factor alpha isoform 2 preproprotein
  • platelet-derived growth factor alpha polypeptidePDGF subunit A
  • platelet-derived growth factor subunit A

Background

The platelet-derived growth factor AA (PDGF-AA) is a member of the PDGF family. The PDGF proteins are derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB). PDGF-AA plays an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in e.coli is a non-glycosylated homodimer of 126 amino acids with a molecular weight of 28.9 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PDGFA
Entrez