Human PDGF-AB ELISA Kit (Colorimetric)

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ELISA: Human PDGF-AB ELISA Kit (Colorimetric) [NBP2-60112]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma and tissue homogenates.
Standard Curve Range
31.25 pg/ml - 2000 pg/ml
Sensitivity
7.8 pg/ml

Order Details

Human PDGF-AB ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human PDGF-AB. No significant cross-reactivity or interference between human PDGF-AB and analogues was observed.
Standard Curve Range
31.25 pg/ml - 2000 pg/ml
Sensitivity
7.8 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Stop Solution
  2. Sample Diluent
  3. Standard (Freeze dried)
  4. Wash Buffer (25 x concentrate)
  5. TMB Substrate
  6. Adhesive Strip (For 96 wells)
  7. Assay plate (12 x 8 coated Microwells)
  8. Biotin-antibody Diluent
  9. Biotin-antibody (100 x concentrate)
  10. Instruction manual
  11. HRP-avidin Diluent
  12. HRP-avidin (100 x concentrate)

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Human PDGF-AB ELISA Kit (Colorimetric)

  • PDGFAB
  • PDGF-AB

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-AB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-AB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-AB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-AB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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