PDE3B Antibody

Western Blot: PDE3B Antibody [NBP1-46184] - Detection of Human PDE3B by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3B antibody NBP1-46184 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PDE3B was also immunoprecipitated by rabbit anti-PDE3B antibody NBP1-46183, which recognizes an upstream epitope. For blotting immunoprecipitated PDE3B, the blot Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds(A) and 10 seconds (B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PDE3B Antibody Summary

• Description: Novus Biologicals Rabbit PDE3B Antibody (NBP1-46184) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1062 and 1112 of human Phosphodiesterase 3B using the numbering given in entry NP_000913.2 (GeneID 5140).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE3B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE3B Antibody

• CGIP1
• CGI-PDE B
• CGIPDE1
• cGMP-inhibited 3'-5'-cyclic phosphodiesterase B
• Cyclic GMP-inhibited phosphodiesterase B
• cyclic nucleotide phosphodiesterase
• EC 3.1.4
• EC 3.1.4.17
• HcGIP1
• PDE3B
• phosphodiesterase 3B, cGMP-inhibited

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE3B