PDCD6 Antibody (2B4) - Azide and BSA Free

Western Blot: PDCD6 Antibody (2B4) [H00010016-M01] - PDCD6 monoclonal antibody (M01), clone 2B4 Analysis of PDCD6 expression in HeLa.

Immunocytochemistry/Immunofluorescence: PDCD6 Antibody (2B4) [H00010016-M01] - Analysis of monoclonal antibody to PDCD6 on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: PDCD6 Antibody (2B4) [H00010016-M01] - Detection limit for recombinant GST tagged PDCD6 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PDCD6 Antibody (2B4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PDCD6 Antibody (2B4) - Azide and BSA Free (H00010016-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-PDCD6 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PDCD6 (AAH12384, 92 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITDWQNVFRTYDRDNSGMIDKNELKQALSGFGYRLSDQFHDILIRKFDRQGRGQIAFDDFIQGCIVLQRLTDIFRRYDTDQDGWIQVSYEQYLSMVFSIV
• Specificity: PDCD6 (2B4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDCD6 Antibody (2B4) - Azide and BSA Free

• ALG2
• ALG-2
• Apoptosis-linked gene 2 protein
• apoptosis-linked gene-2
• FLJ46208
• MGC111017
• MGC119050
• MGC9123
• PDCD6
• PEF1B
• Probable calcium-binding protein ALG-2
• programmed cell death 6
• programmed cell death protein 6

Background

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDCD6 Antibody (H00010016-M01)(3)

Publications using H00010016-M01

• Li X, Rydzewski N, Hider A et al. A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Nat Cell Biol 2016-04-01 [PMID: 26950892] (WB)
• Ilari A, Fiorillo A, Poser E et al. Structural basis of Sorcin-mediated calcium-dependent signal transduction. Sci Rep 2015-01-01 [PMID: 26577048] (ICC/IF)
• Scheffer LL, Sreetama SC, Sharma N et al. Mechanism of Ca(2)(+)-triggered ESCRT assembly and regulation of cell membrane repair. Nat Commun 2014-12-23 [PMID: 25534348] (WB)

Bioinformatics

• Gene Symbol: PDCD6
• Entrez:
  • 10016(Human)
• OMIM:
  • 601057(Human)
• Uniprot:
  • AAH12384(Human)