PDCD4 Antibody (808508) [Janelia Fluor® 646]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 808508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

PDCD4 Antibody (808508) [Janelia Fluor® 646] Summary

• Immunogen: E. coli-derived recombinant human PDCD4
  Lys212-His358
  Accession # Q53EL6
• Specificity: Detects human PDCD4 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD4
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PDCD4 Antibody (808508) [Janelia Fluor® 646]

• H731
• H731nuclear antigen H731
• MGC33046
• MGC33047
• Neoplastic transformation inhibitor protein
• Nuclear antigen H731-like
• PDCD4
• programmed cell death 4 (neoplastic transformation inhibitor)
• programmed cell death protein 4
• Protein 197/15a

Background

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD4