PCPE-1/PCOLCE Antibody (261730)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 261730
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

PCPE-1/PCOLCE Antibody (261730) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PCPE-1
  Gln26-Asp449 (predicted)
  Accession # Q15113
• Specificity: Detects human PCPE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse PCPE-1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: PCOLCE
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PCPE-1/PCOLCE Antibody (261730)

• PCOLCE
• PCPE
• PCPE1
• PCPE-1
• PCPE1Type I procollagen COOH-terminal proteinase enhancer
• procollagen C-endopeptidase enhancer 1
• procollagen C-endopeptidase enhancer
• Procollagen COOH-terminal proteinase enhancer 1
• Procollagen C-proteinase enhancer 1
• procollagen, type 1, COOH-terminal proteinase enhancer
• Type 1 procollagen C-proteinase enhancer protein

Background

Procollagen C‑endopeptidase enhancers, known as PCPEs or PCOLCEs, are secreted extracellular matrix glycoproteins that consist of two CUB domains and one NTR domain. They are known to stimulate enzymatic cleavage of procollagens I ‑ III by the BMP‑1/tolloid family of metalloproteases, also known as procollagen C‑proteinases (1). PCPE‑1 is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin (2). Although BMP‑1/tolloid proteinases are involved in processing of multiple extracellular proteins, the enhancer activity of PCPE‑1 is specific to procollagens since it has no effect on BMP‑1/tolloid cleavage of other substrates (3). It is thought that PCPE‑1 enhances cleavage of procollagens by binding to the substrate and inducing a conformation change in the substrate (3), although interaction between PCPE‑1 and full‑length BMP‑1/tolloid proteinases has also been reported (4).

1. Steiglitz, B.M. et al. (2002) J. Biol. Chem. 277:49820.
2. Kessler, E. et al. (1990) Biochem. Biophys. Res. Commun. 173:81.
3. Moali, C. et al. (2005) J. Biol. Chem. 280:24188.
4. Ge, G. et al. (2006) J. Biol. Chem. 281:10786.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCPE-1/PCOLCE Antibody (MAB2627)(2)

Publications using MAB2627

• P Sansilvest, V Duvivier, F Bertin, N Provost, A Hammoutene, EL Hubert, A Gonzalez, I Tupinon-Ma, V Paradis, P Delerive Procollagen C-Proteinase Enhancer-1 (PCPE-1) deficiency in mice reduces liver fibrosis but not NASH progression PLoS ONE, 2022-02-11;17(2):e0263828. 2022-02-11 [PMID: 35148334] (Western Blot, Human)
• Dongmei Xing, Joseph A. Bonanno Hypoxia reduces TGFbeta1-induced corneal keratocyte myofibroblast transformation Mol Vis 9/11/2009 [PMID: 19753310]

Bioinformatics

• Gene Symbol: PCOLCE
• Uniprot:
  • Q15113()