PBXIP1 Antibody - BSA Free

Immunoprecipitation: PBXIP1 Antibody [NB100-81653] - Detection of Human HPIP/PBXIP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HPIP/PBXIP1 antibody NB100-81653 used for IP at 3 mcg/mg lysate. HPIP/PBXIP1 was also immunoprecipitated by rabbit anti-HPIP/PBXIP1 antibody NB100-81652, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PBXIP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PBXIP1 Antibody - BSA Free (NB100-81653) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human hematopoietic PBX-interacting protein using the numbering given in entry NP_065385.2 (GeneID 57326).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PBXIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2- 5 ug/mg lysate
• Application Notes: This antibody is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PBXIP1 Antibody - BSA Free

• Hematopoietic PBX-interacting protein
• HPIPpre-B-cell leukemia transcription factor interacting protein 1
• pre-B-cell leukemia homeobox interacting protein 1
• pre-B-cell leukemia transcription factor-interacting protein 1

Background

Hematopoietic PBX-interacting protein (HPIP) was identified in a yeast two-hybrid screen for factors that interact with PBX1, a homeodomain protein that regulates gene transcription during development and differentiation. Recently, HPIP has been reported to interact with estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) and modulate ER target gene expression. Alternate names for HPIP include pre-b-cell leukemia transcription factor-interacting protein 1, hematopoietic PBX-interacting protein, and PBKIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBXIP1
• Entrez:
  • 57326(Human)
• Uniprot:
  • NP_065385.2(Human)