PBXIP1 Antibody

Western Blot: PBXIP1 Antibody [NB100-81652] - Detection of Human HPIP/PBXIP1 on HeLa whole cell lysate using NB100-81652. HPIP/PBXIP1 was also immunoprecipitated by rabbit anti-HPIP/PBXIP1 antibody NB100-81653.

Immunohistochemistry-Paraffin: PBXIP1 Antibody [NB100-81652] - Human pancreatic islet cell tumor. Antibody used at a dilution of 1:200.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PBXIP1 Antibody Summary

• Description: Novus Biologicals Rabbit PBXIP1 Antibody (NB100-81652) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human hematopoietic PBX-interacting protein using the numbering given in entry NP_065385.2 (GeneID 57326).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PBXIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. PBXIP1 antibody validated for ICC/IF from a verified customer review. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PBXIP1 Antibody

• Hematopoietic PBX-interacting protein
• HPIPpre-B-cell leukemia transcription factor interacting protein 1
• pre-B-cell leukemia homeobox interacting protein 1
• pre-B-cell leukemia transcription factor-interacting protein 1

Background

Hematopoietic PBX-interacting protein (HPIP) was identified in a yeast two-hybrid screen for factors that interact with PBX1, a homeodomain protein that regulates gene transcription during development and differentiation. Recently, HPIP has been reported to interact with estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) and modulate ER target gene expression. Alternate names for HPIP include pre-b-cell leukemia transcription factor-interacting protein 1, hematopoietic PBX-interacting protein, and PBKIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for PBXIP1 Antibody (NB100-81652) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Siyu Chen IF Rat 09/26/2013

Summary

• Application: Immunofluorescence
• Sample Tested: Rat cortical astrocytes
• Species: Rat

Bioinformatics

• Gene Symbol: PBXIP1
• Entrez:
  • 57326(Human)
• Uniprot:
  • NP_065385.2(Human)