PBK Antibody (3A7)

Western Blot: PBK Antibody (3A7) [H00055872-M07] - Analysis of PBK expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/Immunofluorescence: PBK Antibody (3A7) [H00055872-M07] - Analysis of monoclonal antibody to PBK on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: PBK Antibody (3A7) [H00055872-M07] - Analysis of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml

Western Blot: PBK Antibody (3A7) [H00055872-M07] - Analysis of PBK expression in 293 (Cat # L026V1).

Western Blot: PBK Antibody (3A7) [H00055872-M07] - Analysis of PBK expression in Y-79 (Cat # L042V1).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PBK Antibody (3A7) Summary

• Immunogen: PBK (AAH15191, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGS
• Specificity: PBK (3A7)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PBK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PBK Antibody (3A7)

• Cancer/testis antigen 84
• CT84MAPKK-like protein kinase
• EC 2.7.12.2
• FLJ14385
• Nori-3PDZ-binding kinase
• PDZ binding kinase
• serine/threonine protein kinase
• Spermatogenesis-related protein kinase
• SPKT-LAK cell-originated protein kinase
• TOPKlymphokine-activated killer T-cell-originated protein kinase

Background

This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PBK Antibody (H00055872-M07)(1)

Publication using H00055872-M07

• Warren AY, Massie CE, Watt K et al. A reciprocal feedback between the PDZ binding kinase and androgen receptor drives prostate cancer. Oncogene 2018-09-20 [PMID: 30237440] (WB, Human)

Bioinformatics

• Gene Symbol: PBK
• Entrez:
  • 55872(Human)
• Uniprot:
  • AAH15191(Human)