Paxillin Antibody (GT7612) - Azide and BSA Free

Western Blot: Paxillin Antibody (GT7612) [NBP3-13563] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Paxillin Antibody (GT7612) [NBP3-13563] - Paxillin antibody [GT7612] detects Paxillin protein at focal adhesion sites by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Paxillin Antibody (GT7612) [NBP3-13563] - Paxillin antibody [GT7612] detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Paxillin stained by Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Paxillin Antibody (GT7612) [NBP3-13563] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:1000.

Immunohistochemistry-Paraffin: Paxillin Antibody (GT7612) [NBP3-13563] - Paxillin antibody [GT7612] detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Paxillin stained by Paxillin antibody [GT7612] (NBP3-13563) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: GT7612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Paxillin Antibody (GT7612) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PXN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Paxillin Antibody (GT7612) - Azide and BSA Free

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXN