PAX8 Antibody

Product Discontinued

PAX8 Antibody Summary

• Immunogen: Peptide with sequence C-AQPGSDKRKMDD corresponding to internal region according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.
• Epitope: AQPGSDKRKMDD
• Specificity: This antibody is expected to recognise all reported isoforms (NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAX8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing PAX8A a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  293 Whole Cell Lysate
  PAX8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAX8 Antibody

• paired box 8
• paired box gene 8
• paired box protein Pax-8
• paired domain gene 8

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAX8 Antibody (NB100-59733)(1)

Publication using NB100-59733

• Castro P, Rebocho AP, Soares RJ et al. PAX8-PPAR{gamma} rearrangement is frequently detected in the follicular variant of papillary thyroid carcinoma. J Clin Endocrinol Metab 2005-10-11 [PMID: 16219715]

Bioinformatics

• Gene Symbol: PAX8
• Entrez:
  • 7849(Human)
• Uniprot:
  • NP_003457.1(Human)