PAX8 Antibody

Images

 
Western Blot: PAX8 Antibody [NB100-59733] - Analysis of PAX8 (internal) at 0.5 ug/ml in HEK293 cell lysate (35ug protein in RIPA buffer).
Western Blot: PAX8 Antibody [NB100-59733] - HEK293 overexpressing PAX8A and probed with (mock transfection in first lane).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PAX8 Antibody Summary

Immunogen
Peptide with sequence C-AQPGSDKRKMDD corresponding to internal region according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.
Epitope
AQPGSDKRKMDD
Specificity
This antibody is expected to recognise all reported isoforms (NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: In transfected HEK293 transiently expressing PAX8A a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.
Positive Control
293 Lysate (NB800-PC6)
PAX8 Lysate (NBL1-14132)
Publications
Read Publication using NB100-59733.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAX8 Antibody

  • paired box 8
  • paired box gene 8
  • paired box protein Pax-8
  • paired domain gene 8

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAX8 Antibody (NB100-59733)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAX8
Entrez
Uniprot