PAX8 Antibody

Western Blot: PAX8 Antibody [NB100-1065] - Staining of Human Kidney (A) and HeLa cell (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - Staining of paraffin embedded Human Kidney Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PAX8 Antibody Summary

• Description: Novus Biologicals Goat PAX8 Antibody (NB100-1065) is a polyclonal antibody validated for use in IHC and WB. Anti-PAX8 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence PHNSIRSGHGGLN-C corresponding to N-Terminus according to NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.
• Specificity: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAX8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 - 2 ug/mL
• Application Notes: WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 43.0 kDa band according to NP_039246.1 and 48.2 kDa band according to NP_003457.1).
  IHC-P: A customer has reported unsuccessful use on mouse tissues.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PAX8 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAX8 Antibody

• paired box 8
• paired box gene 8
• paired box protein Pax-8
• paired domain gene 8

Background

This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAX8 Antibody (NB100-1065)(2)

Publications using NB100-1065

• Guenter R, Zheng-Pywell R, Herring B et al. Preclinical safety evaluation of continuous UV-A lighting in an operative setting PloS one 2023-11-17 [PMID: 37976243] (WB, Human)
• Pasca di Magliano M, Di Lauro R, Zannini M. Pax8 has a key role in thyroid cell differentiation. Proc Natl Acad Sci U S A 2000-11-21 [PMID: 11069301]

Bioinformatics

• Gene Symbol: PAX8
• Entrez:
  • 7849(Human)
  • 18510(Mouse)
  • 81819(Rat)
• Uniprot:
  • Q06710(Human)