PAX8 Antibody

Images

 
Western Blot: PAX8 Antibody [NB100-1065] - Staining of Human Kidney lysate
Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - (4ug/ml) staining of paraffin embedded Human Thyroid Gland. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: PAX8 Antibody [NB100-1065] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - IHC analysis of PAX8 in paraffin embedded Human Thyroid Gland using this antibody at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, ...read more
Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - Human Human Thyroid Gland.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PAX8 Antibody Summary

Immunogen
Peptide with sequence PHNSIRSGHGGLN-C corresponding to N-Terminus according to NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.
Specificity
This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 43.0 kDa band according to NP_039246.1 and 48.2 kDa band according to NP_003457.1and). IHC-P: Human thyroid gland shows textured nuclear stains in the epithelial cells around the follicles. An anonymous customer has reported unsuccessful usage on mouse tissues.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PAX8 Lysate (NBL1-14132)
Publications
Read Publication using NB100-1065.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAX8 Antibody

  • paired box 8
  • paired box gene 8
  • paired box protein Pax-8
  • paired domain gene 8

Background

This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAX8 Antibody (NB100-1065)(1)


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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PAX8
Entrez
Uniprot