Pax5/BSAP Antibody (PAX5/2060) - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: PAX5/2060
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Pax5/BSAP Antibody (PAX5/2060) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07549).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human Pax5/BSAP protein (Uniprot: Q02548)
• Localization: Nuclear
• Marker: Early B-Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Pax5/BSAP Antibody (PAX5/2060) - Azide and BSA Free

• B cell specific activator protein
• B-cell-specific transcription factor
• BSAP
• BSAPB-cell lineage specific activator
• paired box 5
• paired box gene 5 (B-cell lineage specific activator protein)
• paired box gene 5 (B-cell lineage specific activator)
• paired box homeotic gene 5
• paired box protein Pax-5
• Pax5
• transcription factor PAX 5

Background

PAX5 encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX5