Pax5/BSAP Antibody (CL14548) - Azide and BSA Free

Analysis in human lymph node and liver tissues using NBP3-44316 antibody. Corresponding Pax5/BSAP RNA-seq data are presented for the same tissues.

Staining of human duodenum shows no positivity in glandular cells as expected.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human chronic lymphocytic leukemia shows moderate nuclear positivity in tumor cells.

Staining of human lymph node shows weak to moderate nuclear positivity in lymphoid cells.

Analysis in human cell line Daudi.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL14548
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Pax5/BSAP Antibody (CL14548) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Pax5/BSAP Antibody (CL14548) - Azide and BSA Free (NBP3-26864) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of Q02548, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Pax5/BSAP Antibody (CL14548) - Azide and BSA Free

• B cell specific activator protein
• B-cell-specific transcription factor
• BSAP
• BSAPB-cell lineage specific activator
• paired box 5
• paired box gene 5 (B-cell lineage specific activator protein)
• paired box gene 5 (B-cell lineage specific activator)
• paired box homeotic gene 5
• paired box protein Pax-5
• Pax5
• transcription factor PAX 5

Background

PAX5 encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX5