Pax5/BSAP Antibody (8F9) - Azide and BSA Free

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 expression in transfected 293T cell line by PAX5 monoclonal antibody (M01), clone 8F9.Lane 1: PAX5 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of monoclonal antibody to PAX5 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml.

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 over-expressed 293 cell line, cotransfected with PAX5 Validated Chimera RNAi ( Cat # H00005079-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAX5 monoclonal antibody (M01), clone 8F9 (Cat # H00005079-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - PAX5 monoclonal antibody (M01), clone 8F9. Analysis of PAX5 expression in IMR-32.

ELISA: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Detection limit for recombinant GST tagged PAX5 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, Mycoplasma
• Clone: 8F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Pax5/BSAP Antibody (8F9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PAX5 (NP_057953, 192 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADTNKRKRDEGIQESPVPNGHSLPGRDFLRKQMRGDLFTQQQLEVLDRVFERQHYSDIFTTTEPIKPEQTTEYSAMASLAGGLDDMKANLASPTPADIGSSVPGPQSYPI
• Specificity: PAX5 - paired box gene 5 (B-cell lineage specific activator)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pax5/BSAP Antibody (8F9) - Azide and BSA Free

• B cell specific activator protein
• B-cell-specific transcription factor
• BSAP
• BSAPB-cell lineage specific activator
• paired box 5
• paired box gene 5 (B-cell lineage specific activator protein)
• paired box gene 5 (B-cell lineage specific activator)
• paired box homeotic gene 5
• paired box protein Pax-5
• Pax5
• transcription factor PAX 5

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX5
• Entrez:
  • 5079(Human)
• OMIM:
  • 167414(Human)
• Uniprot:
  • NP_057953(Human)