Pax5/BSAP Antibody (1H9)


Western Blot: Pax5/BSAP Antibody (1H9) [NBP2-00258] - Daudi cell lysates prepared under reducing conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of Anti-Human/Mouse Pax5 Purified. Bands were more
Flow Cytometry: Pax5/BSAP Antibody (1H9) [NBP2-00258] - Analysis using the PE conjugate of NBP2-00258. Surface staining of C57BL/6 splenocytes with Anti-Mouse CD23 PE-Cy7 and Anti-Mouse CD21/CD35 FITC followed by more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP
0.5 mg/ml

Pax5/BSAP Antibody (1H9) Summary

The immunogen for this antibody is Pax5, BSAP (1H9).
IgG2a Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This 1H9 antibody has been tested by western blot analysis. This can be used at less than or equal to 5ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This 1H9 antibody has also been reported for use in immunoprecipitation and immunohistology staining of frozen tissue sections.

Alternate Names for Pax5/BSAP Antibody (1H9)

  • B cell specific activator protein
  • B-cell-specific transcription factor
  • BSAP
  • BSAPB-cell lineage specific activator
  • paired box 5
  • paired box gene 5 (B-cell lineage specific activator protein)
  • paired box gene 5 (B-cell lineage specific activator)
  • paired box homeotic gene 5
  • paired box protein Pax-5
  • transcription factor PAX 5


The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VHDJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Pax5 expression is correlated with many neoplasms. In diffuse large B cell lymphomas (DLBCL) and non-Hodgkin lymphomas Pax5 is often mutated while in B-cell ALL, expression levels are high. Additionally, translocation with elastin, IGH, ETV6, FOXP1, and EVI3 have been identified.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

PE Labeled NBP2-00257

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Contact Information

Product PDFs


Gene Symbol PAX5

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