Patched 2/PTCH2 Antibody (403438) [Unconjugated]

Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line, WS-1 human fetal skin fibroblast cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB4078) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Patched 2/PTCH2 at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Patched 2/PTCH2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB4078) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 403438
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Patched 2/PTCH2 Antibody (403438) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Patched 2/PTCH2
  Glu79-Glu392
  Accession # Q9Y6C5
• Specificity: Detects human Patched 2/PTCH2 in direct ELISA and Western Blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTCH2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Patched 2/PTCH2 Antibody (403438) [Unconjugated]

• patched (Drosophila) homolog 2
• Patched 2
• patched homolog 2 (Drosophila)
• patched homolog 2
• protein patched homolog 2
• ptc2
• PTCH2

Background

Patched 2 (PTCH2) is a 180-200 kDa 12 transmembrane member of the Patched family. Human PTCH2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa 1115-1203. Together with Smoothened (SMO), PTCH2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Human PTCH2 likely has two isoforms. One shows an eight aa extension of the C-terminus, while a second shows a four aa substitution for the C-terminal 61 amino acids (aa 1143-1203). Over the first extracellular loop human PTCH2 shares 95% and 96% aa sequence identity with mouse and canine PTCH2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTCH2
• Uniprot:
  • Q9Y6C5()