Patched 1/PTCH Antibody [Unconjugated] - (First Extracellular Loop)

Neuro‑2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse Patched 1/PTCH (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4105, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Patched 1/PTCH Antibody [Unconjugated] - (First Extracellular Loop) Summary

• Immunogen: E. coli-derived recombinant mouse Patched 1/PTCH
  Glu108-Asp422
  Accession # Q61115
• Specificity: Detects mouse Patched 1/PTCH in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Patched 1 is observed, and less than 5% cross-reactivity with recombinant mouse Patched-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTCH1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Patched 1/PTCH Antibody [Unconjugated] - (First Extracellular Loop)

• BCNS
• BCNSFLJ42602
• FLJ26746
• HPE7
• mes
• NBCCS
• patched (Drosophila) homolog
• Patched 1
• patched homolog (Drosophila)
• patched homolog 1 (Drosophila)
• patched homolog 1
• protein patched homolog 1
• PTC
• ptc1
• PTCH protein +12b
• PTCH protein +4'
• PTCH protein -10
• PTCH
• PTCH1
• PTCH11

Background

Patched (Ptch1) is a 170‑175 kDa protein that functions in Hedgehog signaling. It is found on multiple cell types and forms a constitutive, inactivating complex with Smoothened. Upon Hedgehog binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Ptch1 has been reported in mice. However, two or more splice forms may exist, with variation occurring over the first 150 aa of the N-terminus. Mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Patched 1/PTCH Antibody (AF4105)(2)

Publications using AF4105

• Kyung, DS;Lee, E;Chae, S;Son, Y;Moon, YJ;Hwang, D;Kim, JK;Lee, YH;Seong, JK; Single-cell transcriptomic analysis reveals dynamic activation of cellular signaling pathways regulating beige adipogenesis Experimental & molecular medicine 2024-10-28 [PMID: 39465352] (Flow Cytometry, Mouse)
• Yaddanapudi K, De Miranda J, Hornig M Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway. PLoS ONE, 2011-10-25;6(10):e26766. 2011-10-25 [PMID: 22046349] (Flow Cytometry, Mouse)

FAQs for Patched 1/PTCH Antibody (AF4105). (Showing 1 - 1 of 1 FAQs).

1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
   • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.

Bioinformatics

• Gene Symbol: PTCH1
• Uniprot:
  • Q61115()