PASD1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PASD1 Antibody [NBP1-84345] - Staining of human cell line PC-3 shows localization to nuclear speckles.

Staining of human skin shows no positivity in squamous epithelial cells as expected.

Staining of human cerebral cortex, liver, skin and testis using Anti-PASD1 antibody HPA011122 (A) shows similar protein distribution across tissues to independent antibody HPA011152 (B).

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Staining of human cerebral cortex shows no positivity in neurons as expected.

Staining of human liver shows no positivity in hepatocytes as expected.

Analysis in human testis and skin tissues using HPA011122 antibody. Corresponding PASD1 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PASD1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QQDPENPVAPLDQAGLMDPVDPEDSVDLGAAGASAQPLQPSSPVAYDIISQELELMKKLKEQLEERTWLLHDAIQNQQNALELMMDHLQKQPNTLRHVVIPDLQSSEAVPKKQQKQHAGQVKRPLPHPKDVKCFCGLSLSNSL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PASD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PASD1 Protein (NBP1-84345PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PASD1 Antibody - BSA Free

• CT63Cancer/testis antigen 63
• OXTES1
• OX-TES-1
• PAS domain containing 1
• PAS domain-containing protein 1

Background

PASD1 may function as a transcription factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PASD1 Antibody (NBP1-84345)(1)

Publication using NBP1-84345

• Djureinovic D, Fagerberg L, Hallstrom B et al. The human testis-specific proteome defined by transcriptomics and antibody-based profiling. Mol Hum Reprod 2014-06-01 [PMID: 24598113]

Bioinformatics

• Gene Symbol: PASD1