Parvalbumin Antibody

Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.

Immunohistochemistry Free-Floating: Parvalbumin Antibody [NBP2-50036] - Analysis of a rat cerebellum section stained with chicken pAb to parvalbumin, NBP2-50036, dilution 1:2,000, in green, and costained with mouse mAb to GFAP, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. NBP2-50036 antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

Parvalbumin Antibody Summary

• Immunogen: Recombinant full length human Parvalbumin. [UniProt# P20472]
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: PVALB
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen
  • Western Blot 1:1000 - 1:5000
• Application Notes: Use in IHC-Fr reported in scientific literature (PMID:33309937). In WB, this antibody recognizes a band at 12 kDa in skeletal muscle lysates which represents parvalbumin and it reacts only with parvalbumin and not the other calcium-binding proteins. This antibody does not recognize parvabumin in rat or mouse brain lysates on western blots.
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
• Preservative: 0.035% Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Parvalbumin Antibody

• D22S749
• MGC116759
• parvalbumin alpha
• parvalbumin
• PV

Background

Parvalbumin is encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Parvalbumin Antibody (NBP2-50036)(6)

Publications using NBP2-50036

• Parmar P, Spahic H, Lechner C et al. Neonatal hypoxia-ischemia alters the events governing the hippocampal critical period of postnatal synaptic plasticity leading to deficits in working memory in mice. Neurobiology of disease 2024-10-30 [PMID: 39486775]
• Spahic H, Parmar P, Miller S et al. Dysregulation of ErbB4 Signaling Pathway in the Dorsal Hippocampus after Neonatal Hypoxia-Ischemia and Late Deficits in PV+ Interneurons, Synaptic Plasticity and Working Memory International Journal of Molecular Sciences 2022-12-28 [PMID: 36613949] (IHC-FrFl, Mouse)
• Mark St Pierre, Neetika Rastogi, Ashley Brown, Pritika Parmar, Charles Lechner, Camille Fung, Raul Chavez-Valdez Intrauterine Growth Restriction Disrupts the Postnatal Critical Period of Synaptic Plasticity in the Mouse Dorsal Hippocampus in a Model of Hypertensive Disease of Pregnancy. Developmental neuroscience 2022-09-14 [PMID: 34933306] (Immunohistochemistry, Mouse)
• Venturino A, Schulz R, De Jesus-Cortes H Et Al. Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain Cell reports 2021-07-06 [PMID: 34233180] (IF/IHC)
• Lechner CR, McNally MA, St Pierre M et al. Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury Neurobiology of disease 2020-12-09 [PMID: 33309937] (IHC-Fr, Mouse)
• Chao O, Fernandes de Velasco E, Pathak S et al. Targeting inhibitory cerebellar circuitry to alleviate behavioral deficits in a mouse model for studying idiopathic autism Neuropsychopharmacology 2020-03-16 [PMID: 32179875] (IF/IHC, Mouse)

FAQs for Parvalbumin Antibody (NBP2-50036). (Showing 1 - 1 of 1 FAQs).

1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
   • We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

Bioinformatics

• Gene Symbol: PVALB
• Uniprot:
  • P20472()
  • P20472()