Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

Western blotting of rat whole brain homogenate with NBP3-52350 (10 µg/ml) and detected with anti-chicken-HRP. This antibody recognizes endogenous Parvalbumin in the rat whole brain homogenate at ~12 kDa.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells and neurons of the molecular layer with NBP3-52350 (visualized in green) at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the Purkinje cells and neurons of the molecular layer in the cerebellum. Magnification 100X.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells and neurons of the molecular layer with NBP3-52350 (visualized in green) at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the Purkinje cells and neurons of the molecular layer in the cerebellum. Magnification 400X.

• Reactivity: Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: L114/81
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Parvalbumin Antibody (L114/81) - Chimeric - BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L114/81 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L114/81 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 1-110 (full-length) of rat Parvalbumin (Uniprot#: P02625) produced recombinantly in E. Coli
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: PVALB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry -Free Floating
  • Immunohistochemistry-Paraffin
  • Western Blot
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

• D22S749
• MGC116759
• parvalbumin alpha
• parvalbumin
• PV

Background

Parvalbumin is encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Parvalbumin Antibody (NBP3-52350). (Showing 1 - 1 of 1 FAQ).

1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
   • We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

Bioinformatics

• Gene Symbol: PVALB