PARC Antibody

Immunohistochemistry-Paraffin: PARC Antibody [NBP1-02783] - Staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Product Discontinued

PARC Antibody Summary

• Immunogen: Synthetic peptide from human PARC corresponding to aa 2503-2517.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL9
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered antiserum
• Preservative: 0.01% Sodium Azide
• Concentration: 85 mg/ml
• Purity: Delipidation and Defibrination

Alternate Names for PARC Antibody

• CUL-9
• cullin 9
• cullin-9
• DKFZp686G1042
• H7AP1DKFZp686P2024
• p53-associated parkin-like cytoplasmic protein
• PARCKIAA0708
• parkin-like cytoplasmic p53 binding protein
• RP3-330M21.2
• UbcH7-associated protein 1

Background

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CUL9
• Uniprot:
  • Q8IWT3(Human)