Papilin Antibody (5B2E5) - BSA Free

Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mouse mAb against Hela (1), HepG2 (2), OCM-1 (3), Raji (4), Jurkat (5), NIH/3T3 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: Papilin Antibody (5B2E5) [NBP2-37320] - Immunofluorescence analysis of NIH/3T3 cells using PAPLN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mAb against human PAPLN recombinant protein. (Expected MW is 36.4 kDa)

Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mAb against HEK293 (1) and PAPLN (AA: 766-870)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Papilin Antibody (5B2E5) [NBP2-37320] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 5B2E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Papilin Antibody (5B2E5) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Papilin (AA: 766-870) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAPLN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 137.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Papilin Antibody (5B2E5) - BSA Free

• Papilin
• Papilin, Proteoglycan-Like Sulfated Glycoprotein
• PAPLN

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAPLN