IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated]


Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL‑28 R alpha /IFN‑ lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by more
Daudi human Burkitt's lymphoma cell line was stained with Human IL‑28 R alpha /IFN‑ lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready

Order Details

View Available Formulations
Catalog# & Formulation Size Price

IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
Accession # Q8IU57
Detects human IL-28 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse
IL‑28 R alpha is observed.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Read Publications using
AF5260 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated]

  • class II cytokine receptor CRF2/12
  • CRF2/12
  • CRF2-12
  • Cytokine receptor class-II member 12
  • Cytokine receptor family 2 member 12
  • IFN-lambda R1
  • IFN-lambda receptor 1
  • IFN-lambda-R1
  • IFNLR1
  • IL-28 R alpha
  • IL-28 receptor subunit alpha
  • IL28R alpha
  • IL-28R1
  • IL28RA
  • IL-28Ra
  • IL-28R-alpha
  • Interferon lambda receptor 1
  • interferon lambda, receptor 1
  • interleukin 28 receptor A
  • interleukin 28 receptor, alpha (interferon, lambda receptor)
  • interleukin 28 receptor, alpha
  • interleukin or cytokine receptor 2
  • interleukin-28 receptor subunit alpha
  • LICR2
  • Likely interleukin or cytokine receptor 2


IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12
(CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the
interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha, respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex
(1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for
IL-28/IL-28 R (7).

  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Meager, A. et al. (2005) Cytokine 31:109.
  3. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  4. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  5. Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409.
  6. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  7. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Bv, Mk, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, ChIP
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow, KO
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ze
Applications: WB, ICC/IF, IHC
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for IL-28R alpha/IFN-lambda R1 Antibody (AF5260)(3)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey).

We have publications tested in 2 applications: Neutralization, Western Blot.

Filter By Application
Western Blot
All Applications
Filter By Species
Primate - Chlorocebus aethiops (African Green Monkey)
All Species

Reviews for IL-28R alpha/IFN-lambda R1 Antibody (AF5260) (0)

There are no reviews for IL-28R alpha/IFN-lambda R1 Antibody (AF5260). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-28R alpha/IFN-lambda R1 Antibody (AF5260) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional IL-28R alpha/IFN-lambda R1 Products

Bioinformatics Tool for IL-28R alpha/IFN-lambda R1 Antibody (AF5260)

Discover related pathways, diseases and genes to IL-28R alpha/IFN-lambda R1 Antibody (AF5260). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on IL-28R alpha/IFN-lambda R1

There are no specific blogs for IL-28R alpha/IFN-lambda R1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol IFNLR1