IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL‑28 R alpha /IFN‑ lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by ...read more
Daudi human Burkitt's lymphoma cell line was stained with Human IL‑28 R alpha /IFN‑ lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
Arg21-Ala228
Accession # Q8IU57
Specificity
Detects human IL-28 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse
IL‑28 R alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
IFNLR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publications using
AF5260 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated]

  • class II cytokine receptor CRF2/12
  • CRF2/12
  • CRF2-12
  • Cytokine receptor class-II member 12
  • Cytokine receptor family 2 member 12
  • IFN-lambda R1
  • IFN-lambda receptor 1
  • IFN-lambda-R1
  • IFNLR
  • IFNLR1
  • IL-28 R alpha
  • IL-28 receptor subunit alpha
  • IL28R alpha
  • IL-28R1
  • IL28RA
  • IL-28Ra
  • IL-28R-alpha
  • Interferon lambda receptor 1
  • interferon lambda, receptor 1
  • interleukin 28 receptor A
  • interleukin 28 receptor, alpha (interferon, lambda receptor)
  • interleukin 28 receptor, alpha
  • interleukin or cytokine receptor 2
  • interleukin-28 receptor subunit alpha
  • LICR2
  • Likely interleukin or cytokine receptor 2

Background

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12
(CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the
interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha, respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex
(1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for
IL-28/IL-28 R (7).

  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Meager, A. et al. (2005) Cytokine 31:109.
  3. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  4. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  5. Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409.
  6. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  7. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF874
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
NBP2-22471
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
NBP2-67168
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37320
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NBP2-75930
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-32905
Species: Hu, Bv, Mk, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF2168
Species: Hu, Mu
Applications: WB, Simple Western, ChIP
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow, KO
NBP2-37584
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-91946
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-80270
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ze
Applications: WB, ICC/IF, IHC
AF5260
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for IL-28R alpha/IFN-lambda R1 Antibody (AF5260)(3)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey).

We have publications tested in 2 applications: Neutralization, Western Blot.


Filter By Application
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
All Species

Reviews for IL-28R alpha/IFN-lambda R1 Antibody (AF5260) (0)

There are no reviews for IL-28R alpha/IFN-lambda R1 Antibody (AF5260). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-28R alpha/IFN-lambda R1 Antibody (AF5260) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional IL-28R alpha/IFN-lambda R1 Products

Bioinformatics Tool for IL-28R alpha/IFN-lambda R1 Antibody (AF5260)

Discover related pathways, diseases and genes to IL-28R alpha/IFN-lambda R1 Antibody (AF5260). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on IL-28R alpha/IFN-lambda R1

There are no specific blogs for IL-28R alpha/IFN-lambda R1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol IFNLR1
Uniprot