IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated]

Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL-28 Ra/IFN-lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-28 Ra/IFN-lambda R1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Daudi human Burkitt's lymphoma cell line was stained with Human IL‑28 R alpha /IFN‑ lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
  Arg21-Ala228
  Accession # Q8IU57
• Specificity: Detects human IL-28 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse
  IL‑28 R alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IFNLR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28R alpha/IFN-lambda R1 Antibody [Unconjugated]

• class II cytokine receptor CRF2/12
• CRF2/12
• CRF2-12
• Cytokine receptor class-II member 12
• Cytokine receptor family 2 member 12
• IFN-lambda R1
• IFN-lambda receptor 1
• IFN-lambda-R1
• IFNLR
• IFNLR1
• IL-28 R alpha
• IL-28 receptor subunit alpha
• IL28R alpha
• IL-28R1
• IL28RA
• IL-28Ra
• IL-28R-alpha
• Interferon lambda receptor 1
• interferon lambda, receptor 1
• interleukin 28 receptor A
• interleukin 28 receptor, alpha (interferon, lambda receptor)
• interleukin 28 receptor, alpha
• interleukin or cytokine receptor 2
• interleukin-28 receptor subunit alpha
• LICR2
• Likely interleukin or cytokine receptor 2

Background

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12
(CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta , CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the
interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha , respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex
(1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for
IL-28/IL-28 R (7).

1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
2. Meager, A. et al. (2005) Cytokine 31:109.
3. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
4. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
5. Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409.
6. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
7. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-28R alpha/IFN-lambda R1 Antibody (AF5260)(4)

Publications using AF5260

• C Xia, JJ Wolf, M Vijayan, CJ Studstill, W Ma, B Hahm Casein kinase 1? mediates degradation of receptors for type I and type II interferons caused by hemagglutinin of influenza A virus J. Virol., 2018-03-14;0(0):. 2018-03-14 [PMID: 29343571] (Western Blot, Human)
• K Saxena, LM Simon, XL Zeng, SE Blutt, SE Crawford, NP Sastri, UC Karandikar, NJ Ajami, NC Zachos, O Kovbasnjuk, M Donowitz, ME Conner, CA Shaw, MK Estes A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infection Proc. Natl. Acad. Sci. U.S.A, 2017-01-09;114(4):E570-E579. 2017-01-09 [PMID: 28069942] (Neutralization, Primate - Chlorocebus aethiops (African Green Monkey))
• Replication of human norovirus RNA in mammalian cells reveals a lack of interferon response J Virol, 2016-09-12;0(0):. 9/12/2016 [PMID: 27466422]
• Wongthida P, Diaz RM, Galivo F Type III IFN interleukin-28 mediates the antitumor efficacy of oncolytic virus VSV in immune-competent mouse models of cancer. Cancer Res., 2010-05-18;70(11):4539-49. 2010-05-18 [PMID: 20484025] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IFNLR1
• Uniprot:
  • Q8IU57(Human)