Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Total protein from human Colon, Thyroid and Stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Panendothelial Cell Antigen (NB100-77668) in blocking buffer and detected with an anti-rat HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).

Immunohistochemistry-Paraffin: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Panendothelial Cell Antigen (MECA-32) was detected in immersion fixed paraffin-embedded sections of human spleen. Antibody at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the endothelial cells.

Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Analysis using the PerCP conjugate of NB100-77668. Staining of bEND.3 cells with MECA-32 AF488.

Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Mouse embryonic cell lysate (A). Antibody at 2 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: MECA-32
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free Summary

• Immunogen: Mouse lymph node stromal cells.
• Specificity: Pan endothelial cells except for CNS & muscle capillaries.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: Plvap
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry <= 0.25 ug/10^6 cells in 100 ul
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500. Use reported in scientific literature (PMID 26586691)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2 ug/ml
• Application Notes: There have been mixed results for use in Immunohistochemisty on paraffin-embedded sections.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

• FELS
• Fenestrated endothelial-linked structure protein
• gp68
• MECA32
• MECA-32
• Panendothelial Cell Antigen
• plasmalemma vesicle associated protein
• Plasmalemma Vesicle Protein 1
• PV-1 protein

Background

MECA-32 is a single-pass type II membrane homodimer glycoprotein (50-55 kDa) primarily expressed on most endothelial cells in embryonic and adult mouse tissues, except in the brain, skeletal, and cardiac muscle, where expression is more constrained. In the brain, it is only expressed in the circumventricular organs and the neurohemal tissues. In skeletal and cardiac muscle, MECA-32 is confined in small arterioles and venules. In cases such as cardiac transplantation or inflammation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to the first 16 days of gestation and disappears thereafter. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Panendothelial Cell Antigen Antibody (NB100-77668)(27)

Publications using NB100-77668

• Stokes ME, Calvo V, Fujisawa S et al. PERK Inhibition by HC-5404 Sensitizes Renal Cell Carcinoma Tumor Models to Antiangiogenic Tyrosine Kinase Inhibitors Clinical cancer research : an official journal of the American Association for Cancer Research 2023-09-21 [PMID: 37733811] (IHC-P, Mouse)
• Kushwaha R, Li Y, Makarava N et al. Reactive astrocytes associated with prion disease impair the blood brain barrier Neurobiology of Disease 2023-09-01 [PMID: 37597815] (Immunohistochemistry-Paraffin, Mouse)
• Laura Trefzer, Maria E Hess, Lena Scholten, Kristin Technau-Hafsi, Frank Meiss, Melanie Boerries, Cristina Has, David Rafei-Shamsabadi Variable Outcome of Immunotherapy in Advanced Multiple Cutaneous Squamous Cell Carcinomas in Two Patients with Recessive Dystrophic Epidermolysis Bullosa. Acta dermato-venereologica 2023-06-21 [PMID: 37338146]
• Wang X, Zhou Y, Sun Q et al. Thymosin ?4 exerts cytoprotective function and attenuates liver injury in murine hepatic sinusoidal obstruction syndrome after hematopoietic stem cell transplantation Transplantation and cellular therapy 2023-05-14 [PMID: 37192732] (IHC, Mouse)
• Prasad R, Adu-Agyeiwaah Y, Floyd JL et al. Sustained ACE2 Expression by Probiotic Improves Integrity of Intestinal Lymphatics and Retinopathy in Type 1 Diabetic Model Journal of clinical medicine 2023-02-23 [PMID: 36902558] (IHC-P, Mouse)
• Xu L, Nirwane A, Xu T et al. Fibroblasts repair blood-brain barrier damage and hemorrhagic brain injury via TIMP2 Cell reports 2022-11-22 [PMID: 36417884] (WB, Mouse)
• Zhu S, Liang J, Zhu F et al. The effects of myeloablative or non-myeloablative total body irradiations on intestinal tract in mice Bioscience reports 2021-03-26 [PMID: 33605406] (IHC-Fr, Mouse)
• Vanslambrouck J M, Wilson S B et al. A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids. J Am Soc Nephrol 2019-01-10 [PMID: 31492807] (IF/IHC, Mouse)
• Noval Rivas M, Wakita D, Franklin MK et al. Intestinal Permeability and IgA Provoke Immune Vasculitis Linked to Cardiovascular Inflammation Immunity 2019-09-17 [PMID: 31471109]
• Fujimoto TN, Colbert LE, Huang Y et al. Selective EGLN Inhibition Enables Ablative Radiotherapy and Improves Survival in Unresectable Pancreatic Cancer Cancer Res. 2019-05-01 [PMID: 31043430] (IHC-P, Mouse)
• Pich C, Meylan P, Mastelic-Gavillet B et al. Induction of paracrine signaling in metastatic melanoma cells by PPARg agonist rosiglitazone activates stromal cells and enhances tumor growth. Cancer Res. 2018-09-05 [PMID: 30185551] (IHC-P, Human)
• KB Jung, H Lee, YS Son, MO Lee, YD Kim, SJ Oh, O Kwon, S Cho, HS Cho, DS Kim, JH Oh, M Zilbauer, JK Min, CR Jung, J Kim, MY Son Interleukin-2 induces the in vitro maturation of human pluripotent stem cell-derived intestinal organoids Nat Commun, 2018-08-02;9(1):3039. 2018-08-02 [PMID: 30072687]
• Wang X, Pan B, Honda G et al. Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain Haematologica 2018-06-14 [PMID: 29903766] (IF/IHC, Mouse)
• Yanai H, Atsumi N, Tanaka T et al. Intestinal stem cells contribute to the maturation of the neonatal small intestine and colon independently of digestive activity Sci Rep 2017-08-31 [PMID: 28860595] (IHC-Fr, Mouse)
• Tarlungeanu DC, Deliu E, Dotter C et al. Impaired Amino Acid Transport at the Blood Brain Barrier Is a Cause of Autism Spectrum Disorder. Cell. 2016-12-01 [PMID: 27912058] (IF/IHC, Mouse)
• Sharmin S, Taguchi A, Kaku Y et al. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation. J. Am. Soc. Nephrol. 2015-11-19 [PMID: 26586691] (IHC-P, Mouse)
• Pippin JW, Sparks MA, Glenn ST et al. Cells of Renin Lineage Are Progenitors of Podocytes and Parietal Epithelial Cells in Experimental Glomerular Disease. Am J Pathol 2013-06-14 [PMID: 23769837] (IF/IHC, ICC/IF, Mouse)
• Pan SC, Wu LW, Chen CL et al. Angiogenin expression in burn blister fluid: Implications for its role in burn wound neovascularization Wound Repair Regen 2012-07-23 [PMID: 22823522] (IF/IHC, Mouse)
• Ma L, Young J, Prabhala H et al. miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Nat Cell Biol. 2010-03-01 [PMID: 20173740] (IF/IHC, Mouse)
• Ran S, Downes A, Thorpe PE. Increased exposure of anionic phospholipids on the surface of tumor blood vessels. Cancer Res. 2002-11-01 [PMID: 12414638] (IF/IHC, ICC/IF, Mouse)
• Mandriota SJ, Jussila L, Jeltsch M et al. Vascular endothelial growth factor-C-mediated lymphangiogenesis promotes tumour metastasis. EMBO J. 2001-02-15 [PMID: 11179212] (IF/IHC, Mouse)
• Tanneau GM, Hibrand-Saint Oyant L, Chevaleyre CC et al. Differential recruitment of T- and IgA B-lymphocytes in the developing mammary gland in relation to homing receptors and vascular addressins. J Histochem Cytochem. 47(12):1581-92. 1999-12-01 [PMID: 10567442] (IHC-Fr, Mouse)
• Wogensen L, Huang X, Sarvetnick N. Leukocyte extravasation into the pancreatic tissue in transgenic mice expressing interleukin 10 in the islets of Langerhans. J Exp Med. 1993-07-01 [PMID: 8100268] (IF/IHC, Mouse)
• Hallmann R et al. Dev. Dyn. 202:325. 1995-01-01 [PMID: 7626790] (IF/IHC)
• Bergese SD et al. Transplantation 57:711. 1994-01-01 [PMID: 7511256] (IF/IHC)
• Leppink DM, Bishop DK, Sedmak DD et al. Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration. Transplantation 48:874. 1989-01-01 [PMID: 2683264]
• Kopp HG, Avecilla ST, Hooper AT,et al. Tie2 activation contributes to hemangiogenic regeneration after myelosuppression. Blood. 106(2):505-13. 2005-07-15 [PMID: 1581767] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: Plvap
• Entrez:
  • 84094(Mouse)
• Uniprot:
  • Q91VC4(Mouse)