Pan PSG Antibody (684701) [Unconjugated]

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PSG Pan Specific Monoclonal Antibody (Catalog # MAB6799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PSG at approximately 72 and 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PSG was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human PSG Pan Specific Mono-clonal Antibody (Catalog # MAB6799) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Product Discontinued

Pan PSG Antibody (684701) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PSG-1
  Val36-Pro419
  Accession # P11464
• Specificity: Detects human PSG-1, -2, -3, -5 and -6 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSG1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pan PSG Antibody (684701) [Unconjugated]

• pan-PSG
• PSG

Background

Pregnancy-specific beta-1-glycoprotein 1 (PSG-1), also called SP1, PSbG1 or B1G1 and designated CD66f, is a 54-72 kDa, 419 amino acid (aa) secreted glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen) superfamily. PSG-1 shares limited aa sequence identity with mouse PSGs, but 84-91% aa sequence identity with human PSG-3, -4, -6, -7, and -8. Potential isoforms of 417-428 aa vary at the extreme C-terminus. PSG-1 is produced by syncytiotrophoblast cells and its concentration increases in maternal plasma as pregnancy progresses. Low plasma PSG-1 in the first or early second trimester has been correlated with fetal growth restriction. Expression of PSGs has also been detected in choriocarcinomas, hydatiform moles, ovarian adenocarcinomas, and breast tumors. During pregnancy, PSG-1 may play role in placental vascular morphogenesis and creation of an anti-inflammatory uterine environment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pan PSG Antibody (MAB6799)(1)

Publication using MAB6799

• Pregnancy Specific ?-1 Glycoprotein 1 is Expressed in Pancreatic Ductal Adenocarcinoma and its Subcellular Localization Correlates with Overall Survival J Cancer, 2016-10-17;7(14):2018-2027. 2016-10-17 [PMID: 27877217] (Western Blot, Human, Mouse)

Bioinformatics

• Gene Symbol: PSG1
• Uniprot:
  • P11464()