PAK [p Thr402] Antibody [Unconjugated]

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately68-70 kDa PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the anti-Phospho-PAK-1, 2, 3 (T402). The immunolabeling of PAK is completely eliminated by treatment with lambda PPase.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PAK [p Thr402] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-T402 of PAK
• Modification: p Thr402
• Specificity: Human, mouse, non-human primates, and rat ~68-70 kDa PAK-1, 2, 3 phosphorylated at T402. T402 in PAK-2 corresponds to T423 in human PAK-1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
• Buffer: 100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAK [p Thr402] Antibody [Unconjugated]

• alpha-PAK;IDDMSSD;Non-specific serine/threonine protein kinase;p65-PAK;PAK1;PAKalpha;Serine/threonine-protein kinase PAK 1
• PAK

Background

PAK (p21-activated kinase) is a general term for a Serine/Threonine kinase that functions downstream of Rho-family GTPases to regulate cytoskeletal (actin) structure and gene transcription. There are two mammalian PAK groups, each with three members. Group I contains PAK-1/ alpha -PAK, PAK-2/ beta -PAK and PAK-3/ gamma -PAK; group II contains PAKs 4-6. All PAKs contain p21-binding domains and a kinase domain. Only group I PAKs are activated by Cdc42 (cell division cycle 24) and exist as dimers. The dimeric form of group I PAKs keep them in an inactive state. This is due to cross-inhibition from one monomer to another. Following p21 binding, these PAKs dissociate and undergo either auto-, or exogenous (PKD1) phosphorylation on a kinase domain Threonine that is embedded in an 18 aa sequence that is absolutely conserved in PAKs 1-3 in both rat and human. In rat, this Threonine occurs at 402 in PAK-2, T422 in PAK-1, and T421 in PAK-3. In human, each position is increased by one (i.e. T403 vs. T402 in rat PAK-2, etc.). Phosphorylation at this homologous site activates the PAKs, which, following additional regulatory phosphorylations, can then interact with desmin, myosin light chain kinase, LIM1, filamin A and/or Merlin. Preparation Prepared from rabbit serum by affinity purification via sequential chromatography

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2. Zkumar, R. et al. (2006) Nat. Rev. Cancer 6:459.
3. Parrini, M.C. et al. (2002) Mol. Cell 9:73.
4. King, C.C. et al. (2000) J. Biol. Chem. 275:41201.
5. Chong, C. et al. (2001) J. Biol. Chem. 276:17347.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.