PAAF1 Antibody (OTI1F11)

Western Blot: PAAF1 Antibody (OTI1F11) [NBP2-46309] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAAF1 .

Western Blot: PAAF1 Antibody (OTI1F11) [NBP2-46309] - Analysis of extracts (35ug) from 9 different cell lines by using PAAF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunofluorescence: PAAF1 Antibody (OTI1F11) [NBP2-46309] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PAAF1.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: OTI1F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PAAF1 Antibody (OTI1F11) Summary

• Immunogen: Full length human recombinant protein of human PAAF1(NP_079431) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:400-4000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAAF1 Antibody (OTI1F11)

• FLJ11848
• PAAF
• proteasomal ATPase-associated factor 1
• Protein G-16
• Rpn14
• WD repeat domain 71
• WD repeat-containing protein 71
• WDR71

Background

PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatorycomplex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotesthe recruitment of 19S regula

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAAF1