PA28 Activator gamma Subunit/PSME3 Antibody

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Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NB100-60557] - (0.1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Concentration
0.5 mg/ml

Order Details

PA28 Activator gamma Subunit/PSME3 Antibody Summary

Immunogen
Synthetic peptide, C-KNIEKIKRPRSSN, representing the C Terminus of the sequence according to NP_005780.2; NP_789839.1
Epitope
KNIEKIKRPRSSN
Specificity
This antibody is expected to recognise both reported isoforms (NP_005780.2 and NP_789839.1).
Clonality
Polyclonal
Host
Goat
Gene
PSME3
Purity
Immunogen affinity purified
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Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
Approx 30kDa band observed in lysates of cell lines Jurkat and K562 (calculated MW of 29.5kDa according to NP_005780.2 and 30.9kDa according to NP_789839.1).

Reactivity Notes

Cross reacts with Human. Expected from sequence similarity: Rat, Dog, Mouse and Cow.

Alternate Names for PA28 Activator gamma Subunit/PSME3 Antibody

  • 11S regulator complex gamma subunit
  • 11S regulator complex subunit gamma
  • Activator of multicatalytic protease subunit 3
  • Ki antigen
  • Ki
  • PA28 Activator gamma Subunit
  • PA28g
  • PA28gamma
  • PA28-gamma
  • PA28GKi nuclear autoantigen
  • proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
  • Proteasome activator 28 subunit gamma
  • proteasome activator 28-gamma
  • proteasome activator complex subunit 3
  • PSME3
  • REG gamma
  • REG-GAMMA

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Product PDFs

Bioinformatics

Gene Symbol PSME3
Entrez
Uniprot

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