PA28 Activator beta Subunit/PSME2 Antibody

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Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB100-59752] - (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PA28 Activator beta Subunit/PSME2 Antibody Summary

Immunogen
Peptide with sequence C-NLEKIVNPKGEEKP corresponding to C-Terminus according to NP_002809.2.
Epitope
NLEKIVNPKGEEKP
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSME2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 27 kDa band observed in human spleen lysates (calculated MW of 27.4 kDa band according to NP_002809.2).
Positive Control
PA28 Activator beta Subunit/PSME2 Lysate (NBL1-14908)
Spleen Lysate (NB820-59259)
Publications
Read Publication using NB100-59752.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PA28 Activator beta Subunit/PSME2 Antibody

  • 11S regulator complex beta subunit
  • 11S regulator complex subunit beta
  • Activator of multicatalytic protease subunit 2
  • MCP Activator
  • MCP activator, 31-kD subunit
  • PA28 Activator beta Subunit
  • PA28b
  • PA28betacell migration-inducing protein 22
  • proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
  • Proteasome activator 28 subunit beta
  • proteasome activator 28-beta
  • proteasome activator complex subunit 2
  • proteasome activator hPA28 subunit beta
  • PSME2
  • REGbeta
  • REG-beta

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PA28 Activator beta Subunit/PSME2 Antibody (NB100-59752)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSME2
Entrez
Uniprot