PA28 Activator beta Subunit/PSME2 Overexpression Lysate (Native)

Images

 
Western Blot: PSME2 Overexpression Lysate (Adult Normal) [NBL1-14908] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSME2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PA28 Activator beta Subunit/PSME2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202431 and based on accession number NM_002818. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens proteasome (prosome, macropain) activator subunit 2 (PA28 beta) (PSME2), mRNA.
Gene
PSME2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PA28 Activator beta Subunit/PSME2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PA28 Activator beta Subunit/PSME2 Overexpression Lysate (Native)

  • 11S regulator complex beta subunit
  • 11S regulator complex subunit beta
  • Activator of multicatalytic protease subunit 2
  • MCP Activator
  • MCP activator, 31-kD subunit
  • PA28 Activator beta Subunit
  • PA28b
  • PA28betacell migration-inducing protein 22
  • proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
  • Proteasome activator 28 subunit beta
  • proteasome activator 28-beta
  • proteasome activator complex subunit 2
  • proteasome activator hPA28 subunit beta
  • PSME2
  • REGbeta
  • REG-beta

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PA28 Activator beta Subunit/PSME2 Lysate (NBL1-14908) (0)

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Bioinformatics

Gene Symbol PSME2
Entrez