| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57628-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06553-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38170-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35372-20ul | |||
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (PSH0-10) [NBP3-32868] - Western blot analysis of PA28 Activator beta Subunit/PSME2 on different lysates with Rabbit anti-PA28 Activator beta Subunit/PSME2 antibody (NBP3-32868) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: 293T cell lysate (10 ug/Lane) Lane 3: Mouse liver tissue lysate (20 ug/Lane) Lane 4: Rat thymus tissue lysate (20 ug/Lane) Lane 5: MCF-7 cell lysate (10 ug/Lane) Lane 6: PC-3 cell lysate (10 ug/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32868) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32868_rabbit-pa28-activator-beta-subunit-psme2-mab-psh0-10-288202412461650.jpg "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (PSH0-10) [NBP3-32868] - Western blot analysis of PA28 Activator beta Subunit/PSME2 on different lysates with Rabbit anti-PA28 Activator beta Subunit/PSME2 antibody (NBP3-32868) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: 293T cell lysate (10 ug/Lane) Lane 3: Mouse liver tissue lysate (20 ug/Lane) Lane 4: Rat thymus tissue lysate (20 ug/Lane) Lane 5: MCF-7 cell lysate (10 ug/Lane) Lane 6: PC-3 cell lysate (10 ug/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32868) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA28 Activator beta Subunit/PSME2 Antibody (PSH0-10) [NBP3-32868] - Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-PA28 Activator beta Subunit/PSME2 antibody (NBP3-32868) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32868) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32868_rabbit-pa28-activator-beta-subunit-psme2-mab-psh0-10-298202414304129.jpg)
![Immunocytochemistry/ Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody (PSH0-10) [NBP3-32868] - Immunocytochemistry analysis of Hela cells labeling PA28 Activator beta Subunit/PSME2 with Rabbit anti-PA28 Activator beta Subunit/PSME2 antibody (NBP3-32868) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA28 Activator beta Subunit/PSME2 antibody (NBP3-32868) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32868_rabbit-pa28-activator-beta-subunit-psme2-mab-psh0-10-29202410234250.jpg)
![Flow Cytometry: PA28 Activator beta Subunit/PSME2 Antibody (PSH0-10) [NBP3-32868] - Flow cytometric analysis of Hela cells labeling PA28 Activator beta Subunit/PSME2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32868, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32868_rabbit-pa28-activator-beta-subunit-psme2-mab-psh0-10-29202416453262.jpg)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of PSME2 expression in transfected 293T cell line by PSME2 monoclonal antibody (M02), clone 1G4.Lane 1: PSME2 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-1G4-Western-Blot-H00005721-M02-img0009.jpg "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of PSME2 expression in transfected 293T cell line by PSME2 monoclonal antibody (M02), clone 1G4.Lane 1: PSME2 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of monoclonal antibody to PSME2 on formalin-fixed paraffin-embedded human stomach carcinoma. Antibody concentration 6 ug/ml.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-1G4-Immunohistochemistry-Paraffin-H00005721-M02-img0005.jpg)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - PSME2 monoclonal antibody (M02), clone 1G4 Analysis of PSME2 expression in MCF-7.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-1G4-Western-Blot-H00005721-M02-img0008.jpg)
![Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of PSME2 transfected lysate using anti-PSME2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSME2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-1G4-Immunoprecipitation-H00005721-M02-img0006.jpg)
![ELISA: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Detection limit for recombinant GST tagged PSME2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-1G4-ELISA-H00005721-M02-img0010.jpg)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP2-57628] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-Western-Blot-NBP2-57628-img0002.jpg "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP2-57628] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody [NBP2-57628] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57628-img0001.jpg)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) [H00005721-M01] - Analysis of PSME2 expression in transfected 293T cell line by PSME2 monoclonal antibody (M01), clone 3F11-1A3.Lane 1: PSME2 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-3F11-1A3-Western-Blot-H00005721-M01-img0004.jpg "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) [H00005721-M01] - Analysis of PSME2 expression in transfected 293T cell line by PSME2 monoclonal antibody (M01), clone 3F11-1A3.Lane 1: PSME2 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) [H00005721-M01] - Analysis of monoclonal antibody to PSME2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-3F11-1A3-Immunocytochemistry-Immunofluorescence-H00005721-M01-img0003.jpg)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB100-59752] - (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-Western-Blot-NB100-59752-img0003.jpg "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB100-59752] - (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-00350] - NBP3-00350 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:31 kDa](http://images.novusbio.com/fullsize/nbp3-00350_rabbit-polyclonal-pa28-activator-beta-subunit-psme2-antibody-235202521451785.png "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-00350] - NBP3-00350 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:31 kDa")
![Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-00350] - PSME2 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg 293T Whole Cell Lysate 2 uL NBP3-00350 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00350, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size: 27 kDa](http://images.novusbio.com/fullsize/nbp3-00350_rabbit-polyclonal-pa28-activator-beta-subunit-psme2-antibody-235202521451773.png)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:30 kDa](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-06-Western-Blot-NBP3-06553-img0001.jpg "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:30 kDa")
![Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-PSME2 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PSME2 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-06-Immunoprecipitation-NBP3-06553-img0002.jpg)
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-38170] - Western Blot analysis of extracts from wild type(WT) and PA28 Activator beta Subunit/PSME2 knockout (KO) 293T(KO) cells, using PA28 Activator beta Subunit/PSME2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38170_rabbit-pa28-activator-beta-subunit-psme2-pab-3112025177022.jpg%3Ft=1690979162 "<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-38170] - Western Blot analysis of extracts from wild type(WT) and PA28 Activator beta Subunit/PSME2 knockout (KO) 293T(KO) cells, using PA28 Activator beta Subunit/PSME2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-35372] - Western Blot analysis of extracts from wild type(WT) and PA28 Activator beta Subunit/PSME2 knockout (KO) 293T(KO) cells, using PA28 Activator beta Subunit/PSME2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35372_rabbit-pa28-activator-beta-subunit-psme2-pab-3112025174442.jpg%3Ft=1690973205 "<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NBP3-35372] - Western Blot analysis of extracts from wild type(WT) and PA28 Activator beta Subunit/PSME2 knockout (KO) 293T(KO) cells, using PA28 Activator beta Subunit/PSME2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: PSME2 Overexpression Lysate (Adult Normal) [NBL1-14908] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSME2.](http://images.novusbio.com/fullsize/PSME2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14908-img0002.jpg "Western Blot: PSME2 Overexpression Lysate (Adult Normal) [NBL1-14908] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSME2.")
![SDS-Page: Recombinant Human PA28 Activator beta Subunit/PSME2 His Protein [NBP1-72527] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human PA28 Activator beta Subunit-PSME2 His Protein-SDS-Page-NBP1-72527-img0002.jpg "SDS-Page: Recombinant Human PA28 Activator beta Subunit/PSME2 His Protein [NBP1-72527] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")