PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33)

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of PSME1 on MCF-7 cell (1) and rat lung tissue (2) lysate using anti-PSME1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Staining PSME1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of paraffin-embedded rat kidney tissue using anti-PSME1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Staining PSME1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Staining PSME1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of paraffin-embedded human tonsil tissue using anti-PSME1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of paraffin-embedded human liver cancer tissue using anti-PSME1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of paraffin-embedded human prostate tissue using anti-PSME1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of paraffin-embedded mouse small intestine tissue using anti-PSME1 antibody. Counter stained with hematoxylin.

Product Discontinued

PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PA28 Activator alpha Subunit/PSME1 aa 1-155 / 249. (SwissProt: Q06323 Human; SwissProt: P97371 Mouse; SwissProt: Q63797 Rat)
• Localization: Cytosol. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PSME1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33)

• 11S regulator complex alpha subunit
• 11S Regulator Complex Subunit alpha
• 29-kD MCP activator subunit
• IFI5111
• IFI5111MGC8628
• IGUP I-5111
• Interferon gamma up-regulated I-5111 protein
• interferon-gamma IEF SSP 5111
• interferon-gamma-inducible protein 5111
• PA28 Activator alpha Subunit
• PA28A
• PA28alphaActivator of multicatalytic protease subunit 1
• proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• Proteasome activator 28 subunit alpha
• proteasome activator complex subunit 1
• proteasome activator subunit-1
• PSME1
• REG alpha
• REGalpha
• REG-alpha

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSME1