p70 S6 Kinase/S6K [p Thr421, p Ser424] Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL of Recombinant Human IGF‑1 (Catalog # 291-G1) for 20 minutes and NIH‑3T3 mouse embryonic fibroblast ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 20 minutes, loaded at 0.2 mg/mL. A specific ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

p70 S6 Kinase/S6K [p Thr421, p Ser424] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human, rat, and mouse p70 S6 Kinase T421/S424 sites
Modification
p Thr421, p Ser424
Specificity
Detects human, mouse and rat p70 S6 Kinase when phosphorylated at T421 and S424 and p85 S6K when phosphorylated at T444 and S447.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KB1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF8965 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p70 S6 Kinase/S6K [p Thr421, p Ser424] Antibody [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases
20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as T389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK1).
  1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
  2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p70 S6 Kinase/S6K Antibody (AF8965)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF8965 Applications Species
T Ueda, Y Kohama, H Sakurai IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A Cell. Signal., 2018;0(0):. 2018 [PMID: 30599213] (Western Blot, Human) Western Blot Human
AE Shay, BT Diwakar, BJ Guan, V Narayan, JF Urban, KS Prabhu IL-4 upregulates cyclooxygenase-1 expression in macrophages J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28684424] (Western Blot, Mouse) Western Blot Mouse
S Sakamoto, M Miyara, S Sanoh, S Ohta, Y Kotake Mild MPP(+) exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation Sci Rep, 2017;7(0):46668. 2017 [PMID: 28443637] (Western Blot, Human) Western Blot Human
Mir, Shakeel, Schwarze, Steven R, Jin, Ling, Zhang, Jinling, Friend, Woodrow, Miriyala, Sumitra, St Clair, Daret, Craven, Rolf J Progesterone receptor membrane component 1/Sigma-2 receptor associates with MAP1LC3B and promotes autophagy. Autophagy, 2013;9(10):1566-78. 2013 [PMID: 24113030] (Western Blot, Human) Western Blot Human
Bradtmoller M, Hartmann C, Zietsch J, Jaschke S, Mautner V, Kurtz A, Park S, Baier M, Harder A, Reuss D, von Deimling A, Heppner F, Holtkamp N Impaired Pten expression in human malignant peripheral nerve sheath tumours. PLoS ONE, 2012;7(11):e47595. 2012 [PMID: 23139750] (Western Blot, Mouse) Western Blot Mouse
Nakai A, Yamaguchi O, Takeda T The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress. Nat. Med., 2007;13(5):619-24. 2007 [PMID: 17450150] (Western Blot, Mouse) Western Blot Mouse

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Blogs on p70 S6 Kinase/S6K.

S6K - a serine/threonine kinase with diverse roles in cell survival and cell cycle progression
S6K is a serine/threonine kinase that is a member of the ribosomal S6 kinase (RSK) family. S6K exists in two main isoforms, S6K1 and S6K2, which can also be alternatively spliced to produce different splice forms. S6K1 has two major splicing produ...  Read full blog post.

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Bioinformatics

Gene Symbol RPS6KB1