p70 S6 Kinase/S6K Antibody (5G9) - BSA Free

Western Blot: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Western blot analysis using S6K S6K mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)

Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Immunohistochemical analysis of paraffin-embedded muscle tissues using S6K mouse mAb with DAB staining.

Flow Cytometry: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Flow cytometric analysis of Jurkat cells using S6K mouse mAb (green) and negative control (red).

Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using S6K mouse mAb with DAB staining.

ELISA: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 5G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

p70 S6 Kinase/S6K Antibody (5G9) - BSA Free Summary

• Description: Novus Biologicals Mouse p70 S6 Kinase/S6K Antibody (5G9) - BSA Free (NBP2-22205) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human p70 S6 Kinase/S6K expressed in E. Coli.
• Localization: Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for p70 S6 Kinase/S6K Antibody (5G9) - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KB1