p70 S6 Kinase beta/S6K2 Antibody - BSA Free

Immunoprecipitation: p70 S6 Kinase beta/S6K2 Antibody [NB100-1544] - Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody NB100-1544 used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody NB100-1545. For blotting immunoprecipitated S6K2, NB100-1545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

p70 S6 Kinase beta/S6K2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Ribosomal Protein S6 Kinase, 70kda, polypeptide 2 using the numbering given in entry NP_003943.2 (GeneID 6199). Tris-citrate/phosphate buffer, pH 7 to 8
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-6 ug/mg of lysate
• Control:

  p70 S6 Kinase beta/S6K2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p70 S6 Kinase beta/S6K2 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• KLS
• p70 ribosomal S6 kinase beta
• p70 S6 Kinase beta
• p70 S6KB
• p70 S6K-beta
• p70(S6K)-beta
• p70-beta
• P70-beta-1
• P70-beta-2
• p70-S6K 2
• P70S6K2
• p70S6Kb
• ribosomal protein S6 kinase beta-2
• ribosomal protein S6 kinase, 70kD, polypeptide 2,70 kDa ribosomal protein S6 kinase 2
• ribosomal protein S6 kinase, 70kDa, polypeptide 2
• RPS6KB2
• S6K2
• S6K-beta
• S6K-beta2
• S6K-beta-2
• serine/threonine-protein kinase 14 beta
• Serine/threonine-protein kinase 14B
• SRK
• STK14B
• STK14BS6 kinase-related kinase

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p70 S6 Kinase beta/S6K2 Antibody (NB100-1544)(1)

Publication using NB100-1544

• Mao Z, LaFortune TA, Alonso MM et al. Cell cycle-dependent nuclear export of phosphatase and tensin homologue tumor suppressor is regulated by the phosphoinositide-3-kinase signaling cascade. Cancer Res 2007-11-01 [PMID: 18006852]

Bioinformatics

• Gene Symbol: RPS6KB2
• Entrez:
  • 6199(Human)
• Uniprot:
  • NP_003943.2(Human)