p70 S6 Kinase beta/S6K2 Antibodies
We offer p70 S6 Kinase beta/S6K2 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each p70 S6 Kinase beta/S6K2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our p70 S6 Kinase beta/S6K2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the p70 S6 Kinase beta/S6K2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our p70 S6 Kinase beta/S6K2 polyclonal antibodies and browse our p70 S6 Kinase beta/S6K2 monoclonal antibody catalog.
Alternate Names for p70 S6 Kinase beta/S6K2 Antibodies
anti-p70 S6 Kinase beta/S6K2 antibody, anti-RPS6KB2 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-KLS antibody, anti-p70 ribosomal S6 kinase beta antibody, anti-p70 S6 kinase beta antibody, anti-p70 S6K-beta antibody, anti-p70 S6KB antibody, anti-p70(S6K)-beta antibody, anti-P70-BETA antibody, anti-P70-beta-1 antibody, anti-P70-beta-2 antibody, anti-p70-S6K 2 antibody, anti-P70S6K2 antibody, anti-p70S6Kb antibody, anti-ribosomal protein S6 kinase beta-2 antibody, anti-ribosomal protein S6 kinase, 70kD, polypeptide 2,70 kDa ribosomal protein S6 kinase 2 antibody, anti-ribosomal protein S6 kinase, 70kDa, polypeptide 2 antibody, anti-S6K-beta antibody, anti-S6K-beta-2 antibody, anti-S6K-beta2 antibody, anti-S6K2 antibody, anti-serine/threonine-protein kinase 14 beta antibody, anti-Serine/threonine-protein kinase 14B antibody, anti-SRK antibody, anti-STK14BS6 kinase-related kinase antibody
8 Results for "p70-s6-kinase-beta-s6k2" in Primary Antibodies
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Western blot analysis of extracts of Mouse brain, using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-5D8F5-Western-Blot-NBP3-16752-img0006.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Western blot analysis of extracts of Mouse brain, using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Confocal imaging of C2C12 cells using p70 S6 Kinase beta/S6K2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025209833.jpg)
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Confocal imaging of NIH/3T3 cells using p70 S6 Kinase beta/S6K2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025208493.jpg)
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Confocal imaging of U-2 OS cells using p70 S6 Kinase beta/S6K2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025209869.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded human cervix cancer tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-8220252010494.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded mouse kidney tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-8220252010403.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded human colon carcinoma tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-8220252010481.jpg)
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry analysis of p70 S6 Kinase beta/S6K2 in paraffin-embedded rat colon tissue using p70 S6 Kinase beta/S6K2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16752_rabbit-p70-s6-kinase-beta-s6k2-mab-5d8f5-822025208423.jpg)
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody (JE35-69) [NBP3-32704] - Western blot analysis of p70 S6 Kinase beta/S6K2 on different lysates with Rabbit anti-p70 S6 Kinase beta/S6K2 antibody (NBP3-32704) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: Jurkat cell lysate, 20 ug/Lane Lane 3: HEK-293 cell lysate, 20 ug/Lane Lane 4: Mouse liver tissue lysate, 40 ug/Lane Lane 5: Mouse kidney tissue lysate, 40 ug/Lane Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32704) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32704_rabbit-p70-s6-kinase-beta-s6k2-mab-je35-69-278202415242626.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody (JE35-69) [NBP3-32704] - Western blot analysis of p70 S6 Kinase beta/S6K2 on different lysates with Rabbit anti-p70 S6 Kinase beta/S6K2 antibody (NBP3-32704) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: Jurkat cell lysate, 20 ug/Lane Lane 3: HEK-293 cell lysate, 20 ug/Lane Lane 4: Mouse liver tissue lysate, 40 ug/Lane Lane 5: Mouse kidney tissue lysate, 40 ug/Lane Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32704) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p70 S6 Kinase beta/S6K2 Antibody (JE35-69) [NBP3-32704] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-p70 S6 Kinase beta/S6K2 antibody (NBP3-32704) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32704) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32704_rabbit-p70-s6-kinase-beta-s6k2-mab-je35-69-298202414304119.jpg)
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (JE35-69) [NBP3-32704] - Immunocytochemistry analysis of HeLa cells labeling p70 S6 Kinase beta/S6K2 with Rabbit anti-p70 S6 Kinase beta/S6K2 antibody (NBP3-32704) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p70 S6 Kinase beta/S6K2 antibody (NBP3-32704) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32704_rabbit-p70-s6-kinase-beta-s6k2-mab-je35-69-2920241192353.jpg)
![Flow Cytometry: p70 S6 Kinase beta/S6K2 Antibody (JE35-69) [NBP3-32704] - Flow cytometric analysis of MCF7 cells labeling p70 S6 Kinase beta/S6K2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32704, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32704_rabbit-p70-s6-kinase-beta-s6k2-mab-je35-69-29202416372135.jpg)
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NBP1-87805] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Western-Blot-NBP1-87805-img0005.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NBP1-87805] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Immunohistochemistry-Paraffin: p70 S6 Kinase beta/S6K2 Antibody [NBP1-87805] - Staining of human esophagus shows nuclear and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Immunohistochemistry-Paraffin-NBP1-87805-img0006.jpg)
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NBP1-87805] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-412](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Western-Blot-NBP1-87805-img0004.jpg)
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody NB100-1545 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Western-Blot-NB100-1545-img0004.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody NB100-1545 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Whole cell lysate (5 and 15 ug) from HeLa cells. Antibody used at 0.04 ug/ml for WB.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Western-Blot-NB100-1545-img0003.jpg)
![Western Blot: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of RPS6KB2 expression in transfected 293T cell line by RPS6KB2 monoclonal antibody (M08), clone 4B11. Lane 1: RPS6KB2 transfected lysatE (53.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-4B11-Western-Blot-H00006199-M08-img0005.jpg "Western Blot: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of RPS6KB2 expression in transfected 293T cell line by RPS6KB2 monoclonal antibody (M08), clone 4B11. Lane 1: RPS6KB2 transfected lysatE (53.4 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of monoclonal antibody to RPS6KB2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-4B11-Immunocytochemistry-Immunofluorescence-H00006199-M08-img0004.jpg)
![ELISA: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Detection limit for recombinant GST tagged RPS6KB2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-4B11-ELISA-H00006199-M08-img0002.jpg)
![Western Blot: p70 S6 Kinase beta/S6K2 [p Ser371] Antibody (S01-4A5) [NBP3-19549] - Western blot detection of Phospho-p70 S6 Kinase beta/S6RK (Ser371) in K562, Rat Brain, C6, 3T3 cell lysates using NBP3-19549 (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-p-Ser371-Antibody-S01-4A5-Western-Blot-NBP3-19549-img0001.jpg "Western Blot: p70 S6 Kinase beta/S6K2 [p Ser371] Antibody (S01-4A5) [NBP3-19549] - Western blot detection of Phospho-p70 S6 Kinase beta/S6RK (Ser371) in K562, Rat Brain, C6, 3T3 cell lysates using NBP3-19549 (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 60kDa.")
![Immunoprecipitation: p70 S6 Kinase beta/S6K2 Antibody [NB100-1544] - Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody NB100-1544 used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody NB100-1545. For blotting immunoprecipitated S6K2, NB100-1545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p70-S6-Kinase-beta-S6K2-Antibody-Immunoprecipitation-NB100-1544-img0001.jpg "Immunoprecipitation: p70 S6 Kinase beta/S6K2 Antibody [NB100-1544] - Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody NB100-1544 used for IP at 3 ug per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody NB100-1545. For blotting immunoprecipitated S6K2, NB100-1545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")