p53 DINP1 Antibody (JG37-64)

Western Blot: p53 DINP1 Antibody (JG37-64) [NBP2-76860] - Western blot analysis of p53DINP1 on different cell lysates using anti-p53DINP1 at 1/500 dilution.Positive control:Line 1: SH-SY-5YLine 2: HepG2Line 3: Mouse stomachLine 3: Human stomach

Immunocytochemistry/Immunofluorescence: p53 DINP1 Antibody (JG37-64) [NBP2-76860] - ICC staining p53DINP1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: p53 DINP1 Antibody (JG37-64) [NBP2-76860] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-p53DINP1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: p53 DINP1 Antibody (JG37-64) [NBP2-76860] - ICC staining p53DINP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: p53 DINP1 Antibody (JG37-64) [NBP2-76860] - Immunohistochemical analysis of paraffin-embedded huamn liver cancer tissue using anti-p53DINP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: p53 DINP1 Antibody (JG37-64) [NBP2-76860] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p53DINP1 antibody. Counter stained with hematoxylin.

Product Discontinued

p53 DINP1 Antibody (JG37-64) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human p53 DINP1 aa 80-188 / 240. (SwissProt: Q96A56 Human; SwissProt: Q9QXE4 Mouse; SwissProt: Q80YE2 Rat)
• Localization: Cytosol. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TP53INP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p53 DINP1 Antibody (JG37-64)

• DKFZp434M1317
• FLJ22139
• p53DINP1
• P53DINP1p53-dependent damage-inducible nuclear protein 1
• p53-inducible p53DINP1
• SIPStress-induced protein
• Teap
• TP53DINP1
• TP53INP1A
• TP53INP1B
• tumor protein p53 inducible nuclear protein 1
• tumor protein p53-inducible nuclear protein 1

Background

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible gene was identified recently and designated p53DINP1 (for p53-dependent damage-inducible nuclear protein and SIP (for stress induced protein) in human and mouse. A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks. p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene encodes two proteins of 27 and 18 kDa in human and mouse termed p53DINP1-a and p53DINP1-b or SIP27 and SIP18. p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP53INP1