p38 alpha [p Thr180, p Tyr182, p Tyr182] Antibody (696602) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-p38a (T180/Y182) Monoclonal Antibody (Catalog # MAB8691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-p38a (T180/Y182) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 696602
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

p38 alpha [p Thr180, p Tyr182, p Tyr182] Antibody (696602) [Unconjugated] Summary

• Immunogen: Phosphopeptide containing the human p38 alpha T180/Y182 site
  Accession # Q16539
• Modification: p Tyr182, p Thr180, p Tyr182
• Specificity: Detects human p38 alpha when phosphorylated at T180 and Y182.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p38 alpha [p Thr180, p Tyr182, p Tyr182] Antibody (696602) [Unconjugated]

• CSBP;CSBP1;CSBP2;CSPB1;EXIP;MAPK14;Mitogen-activated protein kinase;Mitogen-activated protein kinase 14;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
• MAPK14
• p38 alpha
• SAPK2a

Background

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.

The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p38 alpha Antibody (MAB8691)(11)

Publications using MAB8691

• Ge, G;Zhu, B;Zhu, X;Yu, Z;Zhu, K;Cheng, M; Mitochondrial DNA (mtDNA) accelerates oxygen-glucose deprivation-induced injury of proximal tubule epithelia cell via inhibiting NLRC5 Mitochondrion 2024-11-24 [PMID: 39586387] (Western Blot, Mouse)
• M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis Oncogene, 2022-06-14;0(0):. 2022-06-14 [PMID: 35701366] (Simple Western, Xenograft)
• M Lepore Sig, V Grossi, S Di Franco, G Forte, V Disciglio, C Fasano, P Sanese, K De Marco, FC Susca, LR Mangiapane, A Nicotra, G Di Carlo, F Dituri, G Giannelli, G Ingravallo, G Canettieri, G Stassi, C Simone Pharmacological targeting of the novel &beta-catenin chromatin-associated kinase p38&alpha in colorectal cancer stem cell tumorspheres and organoids Cell Death & Disease, 2021-03-25;12(4):316. 2021-03-25 [PMID: 33767160] (Western Blot, Human)
• P Kanmani, A Ansari, J Villena, H Kim Immunobiotics Beneficially Modulate TLR4 Signaling Triggered by Lipopolysaccharide and Reduce Hepatic Steatosis In Vitro J Immunol Res, 2019-03-14;2019(0):3876896. 2019-03-14 [PMID: 31001563] (Western Blot, Human)
• S Simões-Sou, S Littler, SL Thompson, P Minshall, H Whalley, B Bakker, K Belkot, D Moralli, D Bronder, A Tighe, DCJ Spierings, N Bah, J Graham, L Nelson, CM Green, F Foijer, PA Townsend, SS Taylor The p38? Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1? Cell Rep, 2018-10-16;25(3):749-760.e6. 2018-10-16 [PMID: 30332653] (Western Blot, Human)
• Paulraj Kanmani, Hojun Kim Protective Effects of Lactic Acid Bacteria Against TLR4 Induced Inflammatory Response in Hepatoma HepG2 Cells Through Modulation of Toll-Like Receptor Negative Regulators of Mitogen-Activated Protein Kinase and NF-kappa B Signaling Frontiers in Immunology 7/4/2018 [PMID: 30022981]
• X Lei, L Cai, X Li, H Xu, C Geng, C Wang Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model Korean J. Physiol. Pharmacol., 2018-04-25;22(3):269-275. 2018-04-25 [PMID: 29719449] (Western Blot, Human, Mouse)
• Keun Hyung Park, Jiyoung Kim, Eunjoo H Lee, Tae Hoon Lee Cynandione�A inhibits lipopolysaccharide-induced cell adhesion via suppression of the protein expression of VCAM‑1 in human endothelial cells International Journal of Molecular Medicine 1/10/2018 [PMID: 29328438]
• NL Tateosian, JM Pellegrini, NO Amiano, A Rolandelli, N Casco, DJ Palmero, MI Colombo, VE García IL17A augments autophagy in Mycobacterium tuberculosis-infected monocytes from patients with active tuberculosis in association with the severity of the disease Autophagy, 2017-06-05;0(0):14-Jan. 2017-06-05 [PMID: 28581888] (Western Blot, Human)
• Regulatory module involving FGF13, miR-504, and p53 regulates ribosomal biogenesis and supports cancer cell survival Proc. Natl. Acad. Sci. U.S.A, 2016-12-19;0(0):. 2016-12-19 [PMID: 27994142] (Western Blot, Human)
• Photoprotective Effects of Cycloheterophyllin against UVA-Induced Damage and Oxidative Stress in Human Dermal Fibroblasts PLoS ONE, 2016-09-01;11(9):e0161767. 2016-09-01 [PMID: 27583973] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MAPK14
• Uniprot:
  • Q16539()