P2Y2 Antibody (HL2704)

Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.

Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.

Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500.Blue: Fluoroshield with DAPI .

Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: HL2704
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

P2Y2 Antibody (HL2704) Summary

• Description: Novus Biologicals Rabbit P2Y2 Antibody (HL2704) (NBP3-48721) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human P2Y2.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: P2RY2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for P2Y2 Antibody (HL2704)

• ATP receptor
• HP2U
• MGC20088
• MGC40010
• P2RU1
• P2RY2
• P2U nucleotide receptor
• P2U purinoceptor 1
• P2U
• P2U1
• P2UP2UR
• P2Y purinoceptor 2
• P2Y2
• P2Y2P2Y2R
• P2Y2R
• purinergic receptor P2Y, G-protein coupled, 2
• purinergic receptor P2Y2
• Purinergic receptor
• purinoceptor P2Y2

Background

P2Y2 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to both adenosine and uridine nucleotides. It may participate in control of the cell cycle of endometrial carcinoma cells. P2Y2 is also a morphogen receptor that potentiates neurotrophin signaling in neuronal development and regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P2RY2