P2Y11/P2RY11 Antibody (505214) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human P2Y11/P2RY11 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human P2Y11/P2RY11 Monoclonal Antibody (Catalog # MAB9305) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 505214
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

P2Y11/P2RY11 Antibody (505214) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human P2Y11/P2RY11
  Accession # Q96G91
• Specificity: Stains human P2Y11/P2RY11 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P2Y11/P2RY11 Antibody (505214) [Unconjugated]

• P2RY11
• P2Y11
• P2Y11P2Y purinoceptor 11
• purinergic receptor P2Y, G-protein coupled, 11
• purinergic receptor P2Y11

Background

P2RY11 belongs to the family of G protein-coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. P2RY11 is coupled to the stimulation of thephosphoinositide and adenylyl cyclase pathways and behaves as a selective purinoceptor. Naturally occuring read-through transcripts,resulting from intergenic splicing between P2RY11 and an immediately upstream gene (PPAN, encoding peter pan homolog), have been found. Variants in the P2RY11 gene have been associated with narcolepsy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2Y11/P2RY11 Antibody (MAB9305)(1)

Publication using MAB9305

• Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 2019-04-30 [PMID: 31040288] (Assay Development, Human)

Bioinformatics

• Uniprot:
  • Q96G91()