p27/Kip1 Antibody

Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NBP1-32213] - Staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody.

Immunoprecipitation: p27/Kip1 Antibody [NBP1-32213] - Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 ug of p27 Kip1 antibody. Western blot analysis was performed using p27 Kip1 antibody.EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: p27/Kip1 Antibody [NBP1-32213] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (NBP1-32213) diluted at 1:1000.

p27/Kip1 antibody detects p27/Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p27/Kip1 protein stained by p27/Kip1 antibody (NBP1-32213) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 um.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27/Kip1 antibody (NBP1-32213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

p27/Kip1 antibody detects p27/Kip1 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: p27/Kip1 protein stained by p27/Kip1 antibody (NBP1-32213) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p27/Kip1 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit p27/Kip1 Antibody (NBP1-32213) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-p27/Kip1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human p27/Kip1. The exact sequence is proprietary.
• Localization: Nucleus , Cytoplasm
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKN1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (87%), Cat (87%), Pig (84%), Bovine (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for p27/Kip1 Antibody

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p27/Kip1 Antibody (NBP1-32213)(2)

Publications using NBP1-32213

• Lin M, Fang Z, Lin X et al. TRIM55 inhibits colorectal cancer development via enhancing protein degradation of c-Myc Cancer medicine 2023-05-22 [PMID: 37212463] (WB, Human)
• Dickson P, Simanski S, Ngundu JM, Kodadek T Mechanistic Studies of the Multiple Myeloma and Melanoma Cell-Selective Toxicity of the Rpn13-Binding Peptoid KDT-11 Cell Chem Biol 2020-08-26 [PMID: 32857986] (WB)

Bioinformatics

• Gene Symbol: CDKN1B