p27/Kip1 Antibody

Images

 
Western Blot: p27/Kip1 Antibody [NBP1-32213] - Sample (50 ug of whole cell lysate) A: MFB (mouse fibroblast) 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect ...read more
Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody [NBP1-32213] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:500. Red: ...read more
Immunohistochemistry-Frozen: p27/Kip1 Antibody [NBP1-32213] - Frozen sectioned adult mouse retina. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: ...read more
Western Blot: p27/Kip1 Antibody [NBP1-32213] - Sample (50 ug of whole cell lysate) A: CCRF-SB 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: p27/Kip1 Antibody [NBP1-32213] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:1000.
Western Blot: p27/Kip1 Antibody [NBP1-32213] - Whole cell extracts (30 ug) was separated by 12 % SDS-PAGE, and blotted with p27 Kip1 antibody diluted by 1:500
Immunohistochemistry-Frozen: p27/Kip1 Antibody [NBP1-32213] - Staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody.
Immunoprecipitation: p27/Kip1 Antibody [NBP1-32213] - Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 ug of p27 Kip1 antibody. Western blot analysis was performed using p27 Kip1 ...read more

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

p27/Kip1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm
Predicted Species
Monkey (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p27/Kip1 Antibody

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDKN1B
Entrez