p27/Kip1 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

p27/Kip1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: Phosphopeptide containing human p27/Kip1 T198 site
• Specificity: Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p27/Kip1 Antibody [Alexa Fluor® 532]

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27/Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4‑imidazolecarboxamide riboside) or glucose withdrawal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.